优选黄芩提取工艺（职称论文资料）.docVIP

优选黄芩提取工艺（职称论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：优选黄芩提取工艺 1 1 材料与仪器 3 2 方法与结果 3 3 讨论 5 文2：星点设计效应面法优选黄芩提取工艺的研究 6 1 仪器与试药网 http: 7 2 方法与结果网 http: 7 参考文摘引言： 9 原创性声明（模板） 10 文章致谢（模板） 10 正文 优选黄芩提取工艺（职称论文资料） 文1：优选黄芩提取工艺 Optimization Of Extracting Process In Scutellariae Radix Huang Qunlian Tang can 1 Xiao xingquan1 (The Department of Pharmacy In The Hospital Affiliated With Luzhou Medical Pharmacy School of Luzhou Medical College; 1. College of pharmacy，Sichuan luzhou 646000) 【Abstract】 Objective: To research the best extraction conditio of baicalin from Scutellariae Radix by ultrasonic technology and compare each element to determine the best extraction conditio of baicalin; Methods: Ultrasonic technology was used to extract baicalin from Scutellariae Radix， the optimized extraction process was investigated by orthogonal experimental design method， the index was the yielding content of bacicalin which was determined by HPLC;Results: With 60% alcohol as a solvent， the amount of medicine in the 30 times in 20 KHZ frequency ultrasound extracted under three times， each time 60 min， baicalin rate compared to the full.; Conclusion: The ultrasonic technology is a new method of extracting theactive cotitutents in herbs ，which is high extracting efficiency ， stable，reasonable，simple and time-saving，it is worthy of spreading. 【Key words】 ultrasonic extraction method; Scutellaria baicaleis Georgi; Baicalin; optimal method by orthogonal tests；HPLC 双花解毒口服液主要由金银花、黄芩、板蓝根、麦冬、连翘等药物组成，具有清热解毒、利咽止咳等功效，适用于外感风热所致咽喉肿痛、口干咳嗽等症。作为医院制剂在泸州医学院附属医院多年临床应用，疗效确切。黄芩作为双花解毒口服液的臣药，具有清热燥湿、泻火解毒、止血、安胎等功效。研究表明：黄芩具有抗菌抗病毒、抗炎及抗过敏等药理作用，临床上常用于治疗肺热咳嗽、烦渴、热病烦燥、湿热泻痢等疾病[1]。其主要活性成分为：黄芩苷(Baicalin)、黄芩苷元、白杨黄素、汉黄芩素、汉黄芩苷等黄酮类化合物，并以黄芩苷为研究重点。黄芩、黄芩苷不溶于冷水，虽易溶于热水，但煎煮提取冷却后将会出现沉淀，而黄芩、黄芩苷易容于有机溶剂，乙醇可用于双花解毒口服液中黄芩黄芩苷的提取溶剂[2]。为了提高黄芩苷提取率，根据超声波产生的强烈振动、空化、加热、粉碎及搅拌等特性，对黄芩黄芩苷采用超声技术提取。以黄芩苷收率为指标，应用正交设计方法，HPLC测定黄芩苷含量，考察乙醇浓度、药材粒径、超声时间、超声次数四因素三水平优选黄芩超声提取工艺最佳条件。 1 材料与仪器 实验药品与材料 黄芩购自泸州荷花中药饮片有限公司；黄芩苷对照品（0715－9909，