贵州鸡源大肠杆菌分离鉴定及耐药性测定.docVIP

贵州鸡源大肠杆菌分离鉴定及耐药性测定 　　摘 要：经分离培养采自贵州省５个地区县级市65个规模化养鸡场的1 180份样品，生化鉴定得到839株大肠杆菌，分离率为70.89%，采用微量肉汤稀释法测定其对9种抗菌药物的耐药情况。结果表明，分离菌对9种抗菌药物具有不同程度的耐药，土霉素、氟苯尼考和复方新诺明的耐药率高达80%以上，对多粘菌素E、大观霉素耐药率相对较低。839株大肠杆菌均为多重耐药菌株，最少的对2种药物耐药，最多的对9种药物耐药。 　　关键词：大肠杆菌；耐药性；微量肉汤稀释法；最小抑菌浓度 　　中图分类号：S855.1+2 文献标识码：A DOI编码：10.3969/j.issn.1006－6500.2013.02.00８ 　　Identification， Isolation and Antibiotic Resistance of E.Coli from Poultry in Guizhou 　　WANG Guan-yu1， TAN Ai-juan1， LＹU Shi-ming2， ZHANG Jian- yong3 　　（1.College of Ｌife Ｓcience，Guizhou Ｕniversity， Guiyang， Guizhou 550025， China； 2. College of Ａnimal Ｓcience ，Guizhou Ｕniversity，Guiyang， Ｇｕｉｚｈｏｕ 550025， China； 3．Guizhou Province Institute of Veterinary Drug Control， Guiyang， Guizhou 550003， China） 　　Ａｂｓｔｒａｃｔ： To investigate the antibiotic resistance of E. coli isolated from poulrty in large-scale farms of Guizhou. According to the conventional method， 1 180 samples from 65 large-scale farms in five regions of Guizhou were collected， 839 E. coli strains were identified by biochemical identification method， the isolation rate was 70.98%， and were measured the MIC values to 9 kinds of antibiotics with the method of micro-broth dilution. Result demonstrated that the poulrty E. coli strains to the 9 kinds of antibiotics hanve different degrees of resistance， the resistance rates to terramycin， florfenicol and compound sulfamethoxazole were as high as 80% above， the resistance rates to polymyxin E and spectinomycin were relatively low. 839 E. coli strains were multiple drug-resistant strains， and were resistanted to at least to two kinds of antimicrobial， at most to 9 kinds of antimicrobial. 　　Key words： E. coli； antibiotic resistance； micro-broth dilution method； MIC 　　大肠杆菌是医学和兽医临床感染中最常见的病原菌之一[1]，常引起腹泻、尿道感染、腹膜炎，甚至败血症等。在兽医临床上大肠杆菌造成的危害十分严重，它一年四季均可致病，鸡的发病率约为10%-69%，死亡率约为3.8%～72%，给养禽业造成严重经济损失。大肠杆菌病发生后多采用抗生素类药物治疗，使得大肠杆菌得到一定的控制。但是抗生素的选择压力可导致大肠杆菌产生耐药性[2-4]。近年来，随着抗菌药物在养殖业及临床治疗中的广泛应用，大肠杆菌对抗菌药物的耐药状况已相当严重，其耐药性逐年增长[5]。此外，耐药菌株还可通过转化、转导、接合和溶原性转化方式将其携带的耐药基因转移给