绿茶多酚对血管性认知功能障碍影响相关机制探讨.docVIP

绿茶多酚对血管性认知功能障碍影响相关机制探讨 　　 　　 摘要：目的 研究两种剂量的绿茶多酚对慢性脑低灌注后病理生理变化的影响，以探讨绿茶多酚改善血管性认知功能障碍的相关机制。方法 健康雄性Wistar大鼠随机分为假手术组、双侧颈总动脉结扎（2VO）+生理盐水组、2VO+GTP100组［绿茶多酚100 mg/（kg?d）］、2VO+GTP400组［绿茶多酚400 mg/（kg?d）］。采用大鼠2VO制备慢性脑低灌注模型，结扎后从第4周开始给药，给药4周后通过Morris水迷宫观察大鼠的空间学习及记忆功能，采用生化方法检测大鼠海马组织的MDA含量、超氧化物歧化酶（SOD）和乙酰胆碱酯酶（AchE）的活性，并采用实时定量PCR方法检测海马组织Nrf2mRNA的表达。结果 绿茶多酚缩短慢性脑低灌注大鼠的逃逸潜伏期，使大鼠在平台区的停留时间延长。绿茶多酚减少海马组织的MDA含量，增强SOD活性，但对AchE的活性没有显著影响，400 mg/（kg?d）剂量的绿茶多酚增强海??组织Nrf2mRNA的表达。结论 绿茶多酚改善慢性脑低灌注后的认知功能损害，减轻海马组织的脂质过氧化反应及抗氧化能力，尤其是400 mg/(kg?d)的剂量，但对胆碱能系统没有显著影响。 　　 关键词：绿茶多酚；慢性脑低灌注；认知；氧化；乙酰胆碱酯酶 　　 中图分类号：文献标识码：A 文章编号：1672 　　________________________________________ 　　1349（2011）09 　　________________________________________ 　　________________________________________ 　　1672 　　________________________________________ 　　1349(2011)08 　　________________________________________ 　　________________________________________ 　　 　　Effect of Green Tea Polyphenols on Vascular Cognitive Impairment and Its Mechanisms 　　Xu Yan，Zhang Junjian，Yang Ying，et al 　　Department of Neurology，Zhongnan Hospital，Wuhan University (30071 430071) 　　 　　 Abstract：Objective To explore the effect and possible mechanisms of green tea polyphenols（GTP） on vascular cognitive impairment in rats with chronic cerebral hypoperfusion.Methods The male Wistar rats were randomly divided into four groups:Sham operation group，model group，various concentration GTP group administered with GTP 100 mg/（kgod） or GTP 400mg/（kgod）kg/d).The chronic cerebral hypoperfusion models were established by permanent bilateral occlusion of common carotid arteries（2VO）.After model was established，drugs were administered from the fourth week.Spatial learning and memory abilities were evaluated by Morris water maze after the drug were given for 4 weeks.The levels of malondialdehyde (MDA)，the activities of superoxide dismutase (SOD) and acetylcholine esterase (AchE) in the hippocampus were measured biochemically.The mRNA levels of NF 　　____________________