近十年来原位分离技术在生物工程领域的发展课件.pptVIP

* Advances in in-situ product recovery(ISPR) in whole cell biotechnology during the last decade Introduction Advantages of ISPR Requirements Supporting technologies/enabling tools Introduction about this review 1.Reasons The product inhibition and the side reactions lead to substantial downstream processing costs, high waste water volumes, high fermentor costs and an increased substrate cost.so we invest the in-situ product recovery(ISPR) to sove these problems. The rationales a.enrich the product leading to a decrease in downstream processing costs; b. improve the volumetric productivity by alleviation of product inhibition; c. reduce the process flows(decrease amount of waste water per weight unit of product); d.improve the yield by removing the target product from the fermentation broth ISPR is not only used to remove the target product but also used to separate inhibitory by-products. 2.Including of the review The review presents the state-of-the-art in the applications of in-situ product recovery (ISPR) in whole-cell biotechnology over the last 10 years. Several reviews focus on a particular ISPR technique such as pervaporation, extraction, gas stripping,absorption,and ion exchange. All operational modes of cell cultivation (batch, fed-batch and continuous) and all ISPR techniques are considered. Processes using isolated enzymes or plant and animal cell cultures are kept out of scope for this review. 3.Trends in ISPR applications over the last 10 years In the past 10 years, about 140 papers have been published which describe fermentations coupled with ISPR technology. Different configurations of ISPR are possible depending on the location of the separation unit. The selection of the proper ISPR technology are important. a solvents The reviews of ISPR technologies coupled to a fermentation for acetone –butanol –ethanol (ABE) fermentation easily surpassed the reviews o