细胞生物化学实验教材Exp 5-Clinical Biochemistry-zhangjingfa.pptxVIP

Effect of hormones on blood sugar and lipoprotein of normal rats ;Overview; 【Objectives】 （1）To understand the principle of determination of blood sugar and lipid; （2） To understand the effect of hormone on blood sugar and the clinical significance of blood lipid.; 【Principles】 1.1 Blood sugar: glucose in blood. Normal range: 3.89—6.11mmol/L ;1.2 Source and outlet of blood sugar;Epinephrine (adrenaline) is released from nerve endings and the adrenals, and acts directly on the liver to promote sugar production (via glycogenolysis). Epinephrine also promotes the breakdown and release of fat nutrients that travel to the liver where they are converted into sugar and ketones.; Normal blood glucose ;1.5 Ways of determining blood sugar;; 【Principles】 2.1 Blood lipid: lipid in blood. Blood lipids are mainly cholesterol, triglyceride, phospholipid, cholesterol ester and free fatty acid.;Lipoproteins are divided into 4 kinds according to their density, size, electric charge, electrphoresis behavior and immunity. Different lipoproteins have different migration rate in electric field, as follows:; TC (total cholesterol) TG （triglyceride） LDL-C（LDL-cholesterol） HDL-C（HDL-cholesterol）;adult（empty stomach 14~16h） ;;2.5 Blood lipid assay; Triglyceride assay Triglyceride + H2O glycerol+ aliphatic acid Glycerol + ATP Glycerol-3-p +ADP Glycerol-3-p + O2 phosphodihydroxyacetone ;Injecting hormone by subcutaneous injection. Dose: Insulin-0.75IU/kg; Adrenaline-0.2mg/kg; Animal handling Tail bleeding Subcutaneous injection Intraperitoneal injection;Water bath at 37℃ for 10min measuring absorbance at 505nm;Water bath at 37℃ for 10min measuring absorbance at 505nm;Reagent (μl);Blood sugar/lipid sample (mmol/L) = ;Parameters;【Notes】; 【Thought questions】