黄颡鱼和中华绒螯蟹胃肠道形态和功能对投饲响应word格式论文.docx

黄颡鱼和中华绒螯蟹胃肠道形态和功能对投饲的响应中文摘要和菜粕组与对照组差异显著。与对照组相比，饲喂添加水平在 25%的植物蛋白 3 周后，豆粕组中华绒螯蟹 后肠上皮细胞界限模糊，细胞核出现部分缺失；棉粕组和花生粕组核上轻微空泡 化，核排列些微杂乱，结缔组织空洞较多；菜粕组则整个黏膜上皮呈疏松状，损 伤严重。8 周后各组病理情况均有所加重，豆粕组几丁质层有所皱缩；棉粕组部分 上皮不完整，存在的上皮层逐渐形成均质；花生粕组上皮细胞全部呈空泡状，结 缔组织均质化；菜粕组结缔组织与上皮逐渐合为均质。添加水平达到 50%时，各 组在饲喂 3 周时后肠结构就产生明显变化。上述结果表明：黄颡鱼和中华绒螯蟹胃肠道形态结构表现出与之食性相适应 的特点。体质量为 42g 的黄颡鱼和 48g 的中华绒螯蟹能耐受 7d 的饥饿胁迫，且黄 颡鱼在摄食 7d 后各项机能均能恢复正常，但中华绒螯蟹则需要更久的恢复时间。 黄颡鱼和中华绒螯蟹肠道对饲料植物蛋白种类和添加量很敏感，4 种植物蛋白选用 时，花生粕、棉粕、菜粕的安全添加量应在 25%以下，豆粕应在 50%以下，适宜 添加量有待深入研究。关键词： 黄颡鱼；中华绒螯蟹；胃肠道；饥饿；植物蛋白作者：宋霖指导教师：蔡春芳英文摘要黄颡鱼和中华绒螯蟹胃肠道形态和功能对投饲的响应The gastrointestinal tract morphology and function of Pelteobagrus fulvidraco and Eriocheir sinensis as well as its response to feeding and feedAbstractThe gastrointestinal tract is the vital organs for digestion and absorption as well as defense. Normal structure and function of the gastrointestinal tract is very important to the growth and development of economic aquatic animals. Pelteobagrus fulvidraco and Eriocheir sinensis are farmed species developed rapidly in recent years. However, no literature reporting on gastrointestinal tract morphology and its response to feeding and feed of both aquatic animals was found. The experiment first observed gastrointestinal structure under light microscope and characteristics of epithelial ultrastructure of Pelteobagrus fulvidraco and Eriocheir sinensis. On this basis, we studied on the effects of starvation and refeeding on gastrointestinal tract morphology and function. Finally, we investigated the intestinal morphology response to different levels of soybean meal, peanut meal, rapeseed meal and cottonseed meal to provide a theoretical basis for the improvement of two animal feeding and feed formulation.Test 1: we selected Pelteobagrus fulvidraco whose average body weight was 46g and Eriocheir sinensis whose average body weight was 46g as experimental subjects, then quickly killed and dissected. Using light microscopy, scanning electron microscopy and transmission electron micr