btg2基因的肿瘤放射增敏作用的分析word格式论文.docxVIP

Studies on Radiosensitization of Tumor Cells by BTG2 geneAbstractObjectiveThe BTG2 gene is one member of the anti-proliferative BTG/TOB gene family., which is potentially one of the most important target genes in cancer treatment. Recently, there have been no research reports on the influence of BTG2 on tumor radiotherapy. This subject aims at studying the influence of ionizing radiation on BTG2 expression, the function of BTG2 in radiosensitivity of cancer cells and its relevant modifying mechanisms, which will afford an experimental foundation and basis in the subject of BTG2 as a new target gene in tumor radiotherapy.MethodsBreast cancer cell lines MCF-7 and MDA-MB-231, prostate cancer cell line C33A, cervical cancer cell line Hela, non-small cell lung cancer cell line A549 were adopted as the objects of study.Western blot was used to test the effect of ionizing radiation on the expression of BTG2 protein.A pcDNA3-BTG2 expression vector carrying a full length of BTG2 cDNA was successfully constructed and transfected into breast cancer cell lines, MDA-MB-231 and MCF-7. MTT assay and clonogenic forming assay was used to determine the effect of BTG2 expression on tumor radiosensitivity.The combination and interaction of BTG2 and BRCA1.protein was tested by immunoprecipitation.pcDNA3-BRCA1 or siRNA-BRCA1 was used to increase or decrease the expression of BRCA1 in pcDNA3-BTG2 transfected tumor cells, which was aimed at assessing the influence of decreased expression of BRCA1 on the radiosensitizing effect of BTG2.AbstractStudies on Radiosensitization of Tumor Cells by BTG2 gene5）Western blot was used to determine the effect of curcumin on BTG2 proteinexpression in tumor cells.Results137Cs γ irradiation increased the expression of protein expression of BTG2 in tumor cells, including breast cancer cells MCF-7, MDA-MB-231,, prostate cancer cell C33A, cervical cancer cell Hela, lung cancer cell A549, and its increasing level is in a positively dose- dependent manner.Artif