ang 1 vegf在早期糖尿病大鼠视网膜中的表达word格式论文.docxVIP

AbstractObjective:Toexploretheangiopoietin-1(angiopoietin-1,Ang-1)andvascularendothelialgrowthfactor(vascularendothelialgrowthfactor)intheearlyexpressionandsignificancewith theretinaofdiabeticrats.Method:MakesimilarmankindaⅠtypediabetesbigratanimalmodel，Take40SDmaleratdivide the group ofdiabetes 28 and normalmatched control group is 12, The groupofthediabetes 28SDmaleratsintraperitonealinjectiontheSTZ60mgs.Kg-1，aftersettingupmoldsuccess，distinguishinaweek,2weeks,3weeks,4weekstotakethesetofthediabetes7foreachand normal matchedcontrol3toharvessarateyeball,adopttheimmunitymethodexaminationAng-1, VEGFinseetheretina.Result:Ang-1intheretinaofnormalcontrolbigratisnoexpressionin1~4weeks,at1~3weeks, thediabetesbigratretinaseesinnernuclearlayer(INL),ganglioncelllayer(GCL);,andspreadatthebloodvesselcellimmunitytorespondthatthepositivereaction,andthepositivereaction displaypalmtreebesideyellow,alignintheendochylemaofBealesganglioncells,theMǔller cellandjuxtavascularcell,thepositiveexpressionbecomeup-trend,theretinavesselwallblank increasebreadthandretinathicknessbecomethinin4weeksthediabetesbigrat,butafferentappearancehavenoobviouschange,theAng-1positiveexpressionratelowerandhavealreadybecomeanegativeexpression.ThepositiveexpressionrateofAng-1inthefistweekofdiabetes big rat is about70.0%,;the secondweek, the positive expression rate is about 80.0%;thethirdweek, positiveexpressionrateisabout83.3%,thefouthweek,thepositiveexpressionratehasalready changethenegative,andthepositiveexpressionforabout22.2%.ThepositiveexpressionrateofAng-1 in the group of the diabetes 4 weeks ,carries on X2 examinations, X2=21.67,P=0.010(P0.05)andhavecovariancetolearnmeaning.VEGFin1~4weeksnormallybigratmatchedcontrolretinaallisclearthenegativedyesreaction,butin1~4weeks,diabetesmodelgroupretina pathological sectionis clearto align inthebipolar cellofganglion-cell layer（GCL）andspreadatvascularendothelialcell(VEC),theMǔllercellstainingreaction,appearforthe1~3 weeks highexpressionup-trend, the4 weeks, exp