应用lh-pcr对党参麻黄和独一味的内生细菌多样性初探.docVIP

生态环境因子四川松茸菌塘土壤微生物的影响 李小林1，金鑫1,2，李强1,3，熊川1,3，郑林用1,2* 四川省农业科学院土壤肥料研究所成都610066 2四川省农业科学院成都610066 3四川大学生命科学学院成都610065 摘要以四川藏区和攀西松茸代表产区菌塘土壤为研究对象，测定了菌塘土壤土壤微生物量碳氮及土壤酸性磷酸酶、脲酶和过氧化氢酶活性，采用多元回归树分析环境因子对微生物的影响。结果表明：当海拔高于3592 m时，在一定海拔越高~ 3860 m）土壤酶活性；当海拔低于3592 m时，枯枝落叶厚度越厚~ 8.45 cm），海拔越低~ 3226 m）土壤酶活性当坡度大于或等于42.5°时，一定范围内枯枝落叶厚度越高~ 8.45 cm）、海拔越低~ 3225 m），松茸菌塘微生物量及菌落数量越高；而当坡度小于42.5°时，在一定范围内海拔越低~ 3460 m）、枯枝落叶厚度越薄~ 3.2 cm）越有利于松茸菌塘土壤微生物量和菌落数量积累。海拔、坡度及枯枝落叶厚度是影响松茸菌塘土壤微生物活性和生物量的3个关键因素。图表参2关键词：藏区；攀西地区；松茸；微生物活性；微生物量 Effects of ecological environment factor on soil microorganisms of Tricholoma matsutake shiro in Sichuan Province* LI Xiaolin1, JING Xin1,2, LI Qiang1,3, XIONG Chun1,3, ZHENG Linyong1,2* 1Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China 2Sichuan Academy of Agricultural Sciences, Chengdu 610066, China 3School of Life Science, Sichuan University, Chengdu 610065, China Abstract Objectives: In order to understand the effect of microbial activities and biomass on Tricholoma matsutake (Ito et Imai) Sing. shiroes, soil samples of T. matsutake shiroes in Tibetan region and Panxi region of Sichuan Province were collected and investigated, and the potential effects of environmental variables on microorganism of T. matsutake shiroes were discussed. Methods: The microbe quantity of microorganisms (bacteria, actinobacteria, and fungi), the microbial biomass (microbial biomass carbon and nitrogen), and the enzyme activities (acid phosphatase, urease, and catalase) were analyzed. Multivariate regression tree was used to study the microbial activity and microbial biomass associated with environmental variables including elevation, slope, thickness of litter and coverage of the litter. Results: The results indicated that the higher elevation (3841 m ~ 3860 m) increased the enzyme activities when the elevation of T. matsutake shiroes higher than 3592 m, while higher thickness of litter (2.25 cm ~ 8.45 cm) and lower elevation (3058 m ~ 3226