黄金梨果肉硬化症发生关键时期的确定.pdfVIP

ofAnhui 安徽农业科学。Journal A鲥．Sei．201l，39(16)：9562—9564 责任编辑杨莹莹责任校对况玲玲 黄金梨果肉硬化症发生关键时期的确定 于立娜，马春晖’，王然，王永章，李培环 (青岛农业大学园林同艺学院。山东青岛266109) 摘要[目的]确定黄金梨果肉硬化症发生的关键时期。[方法]以黄金梨为材料，研究了黄金梨果实果肩部和果顶部细胞壁组分及其 降解酶活性的变化。[结果]随着黄金梨果实的生长发育．果实纵、横径逐渐增大，果实生长曲线呈单“S”型；果肩和果项部细胞壁组分 及其降解酶活性的变化趋势相似，纤维素和原果胶含量逐渐下降，可溶性果胶含量逐渐上升；纤维素酶(Cx)活性逐渐增高，果胶甲酯酶 生转折，果实膨大期是黄金梨果肉硬化症发生的关键时期；纤维素和Cx可能在果内硬化中起着关键作用。[结论]该研究为果肉硬化症 的防治提供了理论依据。 关键词黄金梨；果肉；细胞壁组分；细胞壁降解酶；果肉硬化症 中图分类号S512．01文献标识码A 文章编号0517—661l(2011)16—09562—03 DeterminationofCritical of SclerosisOccurrenceof period Whangkeumbae Pulp YULi-naet of and 266109) al(DepartmentLandscapeHorticulture，QingdaoAgTiculturalUniversity，Qingdao，Shandong was the disorderoccurrenceof aim to critical ofhard-end Abstract[Objective]Thestudy period infruit was ofcellwall andits activitiesshoulderand studied．[Re— Whangkeumbae鹊material．thechange compenenLsdigestiveenzyme top the and of verticalandhorizontaldiameteroffruitincreased suit]WithgrowthdevelopmentWhangkeumbaefruit，the graduaUy，andshowing ofcellwall andits activitiesinfruitshoulderand were cellulose single…S’type：thechanges componentsdigestiveenzyme top similar，namely and contentreduced soluble contentrose pectin step improvedgraduaHy，while protopeetin gradually，while bystep；cellulose(Cx)activity decreased went andthenwent upfirstly down；solublepectin pectinesterase(PME)activitygradually，andpolygalaeturonase(