nkd1、β―catenin和cyclin d1在结直肠癌中的表达及意义.docVIP

NKD1、β―catenin和Cyclin D1在结直肠癌中的表达及意义 　　[摘要] 目的 ?^察裸角质膜同源蛋白（NKD1）、β-连环素（β-catenin）和细胞周期蛋白D1（Cyclin D1）在结直肠癌组织中的表达，探讨其在结直肠癌变过程中的意义。 方法 收集2014年1月～2016年1月在鄂东医疗集团黄石市中心医院（我院）行结直肠癌根治术切除的癌症组织96例及27例肠管正常黏膜，并收集同期在我院治疗的38例结直肠腺瘤切除标本，采用SP免疫组化法对所有标本中NKD1、β-catenin和Cyclin D1的表达情况进行检测，分析NKD1阳性率、Cyclin D1阳性率与β-catenin异位表达率三者之间的相关性。 结果 结直肠癌组织中NKD1的阳性率（17.71%）明显低于结直肠正常黏膜（92.59%）及腺瘤组织（78.95%），差异有统计学意义（均P 　　[关键词] 结直肠癌；裸角质膜同源蛋白；β-连环素；细胞周期蛋白D1 　　[中图分类号] R735.35 [文献标识码] A [文章编号] 1673-7210（2016）12（b）-0115-04 　　Expression and significance of NKD1， β-catenin and Cyclin D1 in colorectal cancer 　　GU Lei 　　Department of Pathology， Huangshi Central Hospital of Edong Healthcare Group Affiliated Hospital Hubei Polytechnic University， Hubei Province， Huangshi 435000， China 　　[Abstract] Objective To observe the expression of naked cutin membrane homologous protein （NKD1）， β serial element （β- catenin） and cell cycle protein D1 （Cyclin D1） in colorectal cancer tissues and explore its significance in the process of colorectal cancer. Methods 96 cases of colorectal cancer and intestinal resection of the cancer tissue margin of normal mucosa in 27 cases， 38 cases of colorectal adenoma resection specimens in Huangshi Central Hospital of Edong Healthcare Group from January 2014 to January 2016 were collected. SP immunohistochemical method was used to detect the expression of NKD1， beta -catenin and Cyclin D1 in all specimens. The correlation between NKD1 positive rate， Cyclin D1 positive rate and ectopic expression rate of -catenin was analyzed. Results The positive rate of NKD1 in colorectal cancer tissues （17.71%） was significantly lower than that in normal colorectal mucosa and adenoma respectively （92.59%， 78.95%）， the differences were statistically significant （P 　　[Key words] Colorectal cancer； NKD1； β-catenin ； Cyclin D1 　　结直肠癌是临床常见恶性肿瘤，在我国具有较高的发病率和死亡率。研究表明，Wnt信号通路异常活化与结直肠癌病变存在紧密关系[1]。裸角质膜同源蛋白（NKD1）、β-连环素（β-catenin）和细胞周期蛋白D1（Cyclin D1）是Wnt信号通路的重要因子，Wnt异常激活会导致β-catenin异位表达，并在细胞质和细胞核内聚集；NKD