mdig基因在人胰腺癌中的表达及其促癌作用.docVIP

mdig基因在人胰腺癌中的表达及其促癌作用 　　[摘要] 目的 研究mdig基因在胰腺癌细胞中的表达情况，探讨mdig与胰腺癌发生发展的关系及其作用的可能机制。 方法 采用实时荧光定量聚合酶链反应（Q-PCR）和Western blot方法检测mdig在胰腺癌细胞PANC1和MIAPaca2与正常胰腺导管上皮细胞HPDE6c7中表达的差异。将转染mdigsi RNA质粒的胰腺癌细胞PANC1和MIAPaca2设为实验组，将转染空载质粒的胰腺癌细胞PANC1和MIAPaca2设为对照组，用CCK8法和流式测凋亡法分别检测两组细胞的增殖和凋亡情况；用Western blot检测两组细胞的Sox2基因表达情况。 结果 与正常胰腺导管上皮细胞HPDE6c7相比，mdig基因在胰腺癌细胞PANC1和MIAPaca2中表达均上调（P 　　[关键词] 胰腺癌；矿物粉尘诱导基因；增殖；凋亡 　　[中图分类号] R735 [文献标识码] A [文章编号] 1673-7210（2017）03（b）-0012-04 　　Expression of mdig gene in human pancreatic carcinoma and its role in promoting cancer 　　LUO Hesheng1 ZHAN Ting1 TIAN Xia HUANG Xiaodong2 　　1.Department of Gastroenterology， Peoples Hospitial of Wuhan University， Hubei Province， Wuhan 430060， China； 2. Department of Gastroenterology， Tongren Hospital of Wuhan Univerity， Hubei Province， Wuhan 430060， China 　　[Abstract] Objective To study the expression of mdig in pancreatic cancer cells， in order to explore the relationship between mdig and the development of pancreatic cancer and its possible mechanism. Methods The expression of mdig in pancreatic cancer cells and normal pancreatic ductal epithelial cells were detected by Q-PCR and Western blot. The pancreatic cancer cells PANC1 and MIAPaca2 transfected with mdigsi RNA plasmid were designated as the experimental group， and the pancreatic cancer cells PANC1 and MIAPaca2 transfected with empty plasmid were designated as control group， the proliferation of two groups of cells was detected by CCK8， the apoptosis of two groups of cells was detected by flow cytometry， the expression of Sox2 in two groups of cells was detected Western blot. Results Compared with normal pancreatic ductal epithelial cells HPDE6c7， the expression of mdigin pancreatic cancer cells PANC1 and MIAPaca2 was up-regulatede. Compared with the control group， the proliferation rate of the experimental group was decreased （P 　　1 材料与方法 　　1.1 细胞系及细胞培养 　　人胰腺癌细胞PANC1、MIAPaca2以及人正常胰腺导管细HPDE6c7（H6c7）购自中国科学院上海细胞库。细胞用含10%胎牛血清（NQBB，USA）、1%双抗（Thermo Fish