电化学发光法定量检测乙型肝炎表面抗原与荧光定量PCR检测乙肝病毒DNA载量的关系.docVIP

精品论文 参考文献 电化学发光法定量检测乙型肝炎表面抗原与荧光定量PCR检测乙肝病毒DNA载量的关系 黄振勇 罗永钊（暨南大学医学院第五附属医院清远市人民医院 广东清远 511500） 【中图分类号】R512.6+2【文献标识码】A【文章编号】1672-5085（2012）17-0028-03 【摘要】目的 探讨外周血中电化学发光免疫分析法(ECLIA)定量检测乙型肝炎表面抗原(HBsAg)与荧光定量PCR检测乙肝病毒DNA(HBV-DNA)载量之间的相互关系。方法 运用电化学发光法、荧光定量PCR法两种方法同时检测180份血清标本的HBsAg含量和HBV-DNA载量，并按HBV-DNA载量分成4组，对其结果应用统计学分析。结果 在180份血清标本中，HBsAg阳性为161例，占89.4%，HBV-DNA阳性为101例，占56.1%，两者同时为阳性的有101例，占56.1%。经统计学分析HBsAg定量与HBV-DNA载量之间无统计学意义，没有相关性（r＜0.3，P＞0.05）。结论 电化学发光法定量检测HBsAg与HBV-DNA载量无关，联合检测两者可以为临床提供诊断治疗依据。 【关键词】乙型肝炎表面抗原(HBsAg) HBV-DNA 电化学发光免疫分析法(ECLIA) 荧光定量PCR(FQ-PCR) The correlation between quantitative detection of Hepatitis B surface antigen by Electrochemi luminescene and Fluorescence quantitative PCR detection of Hepatitis B virus DNA load HUANG Zhen-yong ，Luo yong-zhao （The Fifth Affiliated Hospital of Medical College Jinan University， Qingyuan city Peoplersquo;s HospitalGuangdong Qingyuan 511500） 【Abstract】Objective To explore the correlation between quantitative detection of Hepatitis B surface antigen (HBsAg) by Electrochemi luminescene immunoassay (ECLIA) and Fluorescence quantitative PCR detection of Hepatitis B virus DNA (HBV-DNA) load in peripheral blood. Method Serum samples from 180 patients were measured simultaneously the quantitative of HBsAg and HBV-DNA with Electrochemi luminescence immunoassay and Fluorescent quantitative PCR, Pressing HBV-DNA to load into 4 groups all attained data were then undergone statistical analysis. Result In the 180 serum samples,HBsAg was positive in 161 cases, accounting for 89.4%, HBV-DNA was positive in 101 cases, accounting for 56.1%, both positive in 101 cases, accounting for 56.1% .The relevant analysis is no statistical significance between HBsAg and HBV-DNA（r＜0.3，P＞0.05）. Conclusion That is no correlation between the quantitative HBsAg and HBV-DNA load, Combined detection of both can be provided for clinical in diagnosis and treatment of foundation. 【Key words】HbsAg HBV-DNA Electrochemi luminescence imm