瑞舒伐他汀对ox-LDL诱导的TF表达的影响及机制研究.docVIP

精品论文 参考文献 瑞舒伐他汀对ox-LDL诱导的TF表达的影响及机制研究 吕慎昌 颜新峰 高爱玲 　　（山东省莱芜市妇幼保健院 山东 莱芜 271100） 　　【摘 要】目的：探讨瑞舒伐他汀对氧化修饰低密度蛋白（ox-LDL）诱导的人单核-巨噬细胞组织因子（TF）表达的影响机制。方法：采用体外密度离心法分离健康人单核细胞培养并培养到第4代，采用佛波脂（160nmol/L）对巨噬细胞进行诱导24h后用于实验，根据实验要求分为空白组、ox-LDL组、瑞舒伐他汀组，采用逆转录聚合酶链式反应（RT-PCR）测定各组的TFmRNA、TF蛋白、人单核-巨噬细胞（LOX-1mRNA）的表达情况。结果：TF基因的RT-PCR产物溶解曲线温度峰值为82.5℃，溶解曲线未见任何切迹，曲线光滑锐利，证实TF基因扩增产物特异性强，无异常扩增现象。三组实验标本的LOX-1mRNA、TFmRNA、TF蛋白表达差异显著（Plt;0.05）；组间两两比较，LOX-1mRNA、TFmRNA、TF蛋白在三组间的差异为ox-LDL组gt;瑞舒伐他汀组gt;空白组（Plt;0.05）。结论：瑞舒伐他汀可以通过下调TFmRNA、LOX-1mRNA表达水平从而达到TF蛋白的表达，可以有效降低急性冠脉事件的发生率。 　　【关键词】瑞舒伐他汀；氧化修饰低密度蛋白；人单核-巨噬细胞组织因子；影响机制 　　【中图分类号】R541.4 【文献标识码】A 【文章编号】1004-6194（2015）01-0001-02 　　Study on the effect of rosuvastatin on expression of ox-LDL induced by TF and its mechanism 　　【Abstract】Objective: Study of rosuvastatin on oxidized low density lipoprotein(ox-LDL) induced human monocyte - macrophage tissue factor (TF) expression of the impact mechanism. Methods: Isolation and culture of health human mononuclear cellsand cultured to the fourth generation of in vitro usingdensity centrifugation, by phorbol ester (160nmol/L) onmacrophages were used for the experiment after the induction of 24h, according to the experimentalrequirements are divided into blank control group, ox-LDL group, rosuvastatin, reverse transcription polymerasechain reaction (RT-PCR) were measured TFmRNA, TFprotein, human monocyte macrophages (LOX-1mRNA)expression. Results: The product of RT-PCR TF gene of the dissolution curve of the peak temperature is 82.5 DEG C, any notch no dissolution curve smooth, sharp, confirmed that the TF gene amplified product specificity, no abnormal amplification phenomenon. The three groups of the experimental specimens of LOX-1mRNA, TFmRNA, TF protein expression were significantly difference (Plt;0.05);the comparison between two two groups, the difference of LOX-1mRNA, TFmRNA, TF protein in three groups ox-LDL group gt; rosuvastatin group gt; blank group (Plt;0.05). Conclusion:Rosuvastatin can through t