elisa法和eclia法检测乙肝五项的结果分析.docVIP

精品论文 参考文献 ELISA法和ECLIA法检测乙肝五项的结果分析 刘志洲 吕波 刘杨 张晓雷 郑俞 杨鹏（通讯作者） 　　（解放军第210医院403临床部 辽宁 大连 116021） 　　【摘要】 目的：分析酶联免疫吸附试验（ELISA）和电化学发光技术（ECLIA）检测乙肝五项的结果差异。方法：分别用ELISA法和ECLIA法测定107例乙肝病人血清标本的HBsAg、HBsAb、HBeAg、HBeAb、HBcAb，检测结果用阴性和阳性来表示，并用chi;2检验进行统计学分析。结果：两种方法对于HBsAg、HBsAb、HBeAg、HBeAb检测结果无统计学差异，对于HBcAb检测结果有显著性差异。结论：ECLIA法检测灵敏度可能高于ELISA法，有条件的实验室应尽量采用ECLIA法检测乙肝五项。 　　【关键词】 乙肝；酶联免疫吸附试验；电化学发光技术 　　【中图分类号】R446 【文献标识码】A 【文章编号】2095-1752（2016）24-0081-02 　　 　　Analysis the Results of Detecting Five Items of Hepatitis B Serum Markers by ELISA and ECLIA 　　Liu Zhizhou, Lv Bo, Liu Yang, Zhang Xiaolei, Zheng Yu, Yang Peng. 　　403 Clinical Department, 210 Hospital of PLA, Dalian 116021 　　【Abstract】 Objective To analysis the results of detecting five items of serum markers for Hepatitis B by ELISA and ECLIA. Methods A total of 107 Hepatitis B patients serum samples were randomly selected to test five items of hepatitis B serum markers, using enzyme linked immunosorbent assay(ELISA) and electrochemiluminescence immunoassay(ECLIA) separately．There were Hepatitis B virus surface antigen(HBsAg)，Hepatitis B virus surface antibody(HBsAb)，Hepatitis B virus e antigen(HBeAg)，Hepatitis B virus e antibody(HBeAb) and Hepatitis B virus core antibody(HBcAb)．The detecting results were described by negative and positive. Date was statistically analysed by chi;2 test. Results There are no statistical difference for the detecting results of HBsAg,HBsAb,HBeAg,HBeAb between two methods. Whereas, there is significant difference for the detecting results of HBcAb . Conclusions The detectability of ECLIA is higher than ELISA, and ECLIA maybe the more appropriate test for detecting five items of serum markers for Hepatitis B. 　　【Key words】 Hepatitis B; ELISA; ECLIA 　　我国是乙型肝炎病毒(HBV)感染人数最多的国家，HBV携带者约占总人口的10%左右[1]。乙型肝炎病毒的血清学标志物检测是医院检验科的常规检验项目，可以了解人体对乙型肝炎病毒的免疫力、是否具有传染性、评估乙型肝炎病毒复制的活跃程度、判断疾病转归和预后等。最常用的乙型肝炎病毒的血清学标志物是乙肝五项，包括乙肝表面抗原（HBsAg）、乙肝表面抗体（HBsAb）、乙肝e抗原（HBeAg）、乙肝e抗体