人嗜碱性粒细胞肿瘤细胞系KU812F检测牛乳及其替代品致敏性方法.PDFVIP

202 2011, Vol. 32, No. 14 食品科学 ※分析检测 KU812F 1 2, 丛艳君 ，任发政 * (1.北京工商大学食品学院，食品添加剂与配料北京高校工程研究中心，北京市食品风味化学重点实验室，北京 100048；2. 中国农业大学食品科学与营养工程学院，北京 100083) 比较牛乳及其替代品释放组胺的能力，以建立体外细胞检测牛乳及替代品疑似过敏原组分致敏性的检测方 法。以嗜碱性粒细胞 KU812F 为研究对象，通过牛乳过敏患者血清的孵育，使细胞处于致敏状态，再以牛乳及其 替代品过敏原作为激发物，使细胞脱颗粒释放组胺。结果表明：利用嗜碱性粒细胞 KU812F 检测牛乳及替代制品 组胺释放量的方法具有较高的可靠性及重现性，细胞释放组胺的量与过敏原的剂量相关，在一定的剂量范围内，组 胺的释放率与过敏原的剂量呈正比，组胺释放率达到最大之后，组胺释放率与剂量呈负相关。 KU812F ；组胺；过敏反应；牛乳粉 Construction of an in vitro Allergy Reaction Evaluation Method for Cow Milk and Its Substitutes Using Human Leukemia Cell Line KU812F 1 2, CONG Yan-jun ，REN Fa-zheng * (1. Beijing Key Laboratory of Food Flavor Chemistry, Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China； 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) Abstract ：This study aimed to construct an in vitro cellular assay for suspected allergenic components of cow milk and its substitutes using human leukemia cell line KU812F, an immature prebasophillic cell line based on their histamine release ability. KU812F was differentiated into basophils by incubation with serum from cow milk allergic people. The release of histamine from differentiated KU812F cells was monitored after sensitization with either cow milk or its substitutes. The results showed that this in vitro assay was highly reliable and reproducible in determ