西维来司钠抑制裸鼠肺癌H460细胞胸腔种植瘤生长和转移的机制.pdfVIP

现代肿瘤医学 2010年 08月 第 l8卷第 08期 西维来司钠抑制裸鼠肺癌 H460细胞胸腔种植瘤生长和转移的机制 朱运奎，郭晓斌，李继东，刘 卫，马国成 Mechanism ofONO一5046．Nainhibitingtumorgrowthandmetastasisoflungcancerin athymicmouse ZHU Yun—kui，GUOXiao—bin，LIJi—dong，LIUWei，MA Guo—cheng DepartmentofRespiratoryDisease，LanzhouGenerlaHospital，LanzhouCommandPLA ，Lanzhou730050，China． 【Abstract】 0bjective：ToevaluatetheinhibitionofONO一5046．Naontumorgrowthandmetastasisoflungcareer ofthethoraciccavityimplantmodelinathymicmouse．M ethods：H460cellswereinoculatedsubcutaneouslyintothe thoraciccavityofathymicmouse，andthemousewererandomizedintoexperimentalgroupandcontrolrgoup ．Inthe experimentalgroup，ONO一5406．Nawasinjectedintraperitoneallyatadoseof50mgdailyfromdaylaftertumorin— oculation，thecontrolgroupwastreateddailywithequalvolumeofPBSfor28days．Transplantationtumorweights，the rateoftumormetastasis，micelife span，MMP 一2ofbloodwereobserved．Results：Therateofformingtumorwas 100％ ，andthethoraciccavityimplantmoelsgraduallyappearedmetastases．Thetransplantationtumorweightsofex— perimentalrgoupwerelessthanthatofcontrolgroup ；Theaveragesurvivalperiodsofexperimentalgroupswaslonger thanthatofcontrolgroup．TheexpressionofMMP一2ofexperimentalrgoup bloodwaslowerthanthatofcontrol group．Conclusion：TheseresultsdemonstratethatONO一540 6．NacouldinhibitrgowthandmetastasisofH460cell inathymicmouse，andoneoftheimportantmechanismsinvolvesininhibitionoftheMMP一2expression，thethoracic cavityimplantmodelwaseasertocausemetastsis，andprovidedareliableresearchtoolforstudyingthemechanisms ofONO一5046．Nainhibitingtumorrgowthandmetastasisoflungcancer． K【eywords】lungcarcinomamodel；ONO一5046．Na；matrixmetalloproteinase一2；gelatinasezymorgaphy ModernOucology2010，18(O8)：1485—1487 【摘要】 目的：建立裸鼠肺癌胸腔移植模型，观察西维来司钠 (SivelestatSodium或 ONO一5406，Na)对裸鼠肺 癌生长和转移的抑制作用。方法：将H460大细胞肺癌接种于裸鼠左侧胸腔 ，并随机分成实验组和对照组。