干细胞实验研究中的生物安全风险及其防控对策.PDF

2011 1 35 1 M ilM ed c,i Vo l35, No 1, Jan, 2011 17 论 著 1 2 1 , , [ ] 干细胞生物学是 20世纪末和本世纪初生命科学最令人瞩目的研究领域之一, 它的研究几 涉及了所有 生命科学和生物医药学领域, 尤其对再生医学的发展及其在遗传代谢性疾病或创伤修复治疗中的应用具有重要意 义在干细胞的基础与应用研究飞速发展的同时, 干细胞的体外培养和实际应用中可能出现的安全问题同样引起 了广泛的关注文章分析了导致干细胞污染的可能途径, 探讨了针对不同污染途径所进行的防控措施, 为我国干 细胞研究与应用的实施提供参考 [ ] 干细胞; 内源性污染; 外源性污染; 防控; 细胞培养技术; 实验室感染; 再生医学 [ ] R457. 7 [] A [ ] 2011) B iosecu rity and its con trol strategies in stem cell experim ental research 1 2* 1* WAN G Zh uang , L I J insong , PE I X uetao ( 1. M ilitary Key Laboratory of tem Cell and Regenerative M ed icine, Beijing Institute of Transfusion M edicine, Beijing 100850, Ch ina; 2. tateKey Laboratory of Pathogen and B iosecu rity, Institute ofM icrob iology and Ep idem iology, Academy ofM ilitaryM edical ciences, B eijing 100071, China) * Corresponding author, L I Jinsong, Tel: 01066948559, Em ail: L ijs@ 163. com; PE I Xuetao, Tel: 01066931980, Em ail: peixt@ nic. bm i. ac. cn [ A bstract] tem cell biology is one of the hottest spheres of research in life science around the turn of th is Century. Its research involves alm ost each field of life sciences and biom edicine, especially mi portant in the developm ent of regenerative m ed icine and its app lication in treatm ent of genetic metabolic diseases orw ound healing. H owever, biosecurity issues of stem cells during app lication m ay attract w ide atten tion. Th is article analyzes the possible causes of the contam ination of stem cells, and exp lores prevention and control coun term easu res for d ifferen t causes so as to provide references for the stem cell research and app licat