稀有人参皂苷对试验性白癜风模型的影响-国家级大学生创新创业训练.pdfVIP

稀有人参皂苷对实验性白癜风模型的影响 西北大学 吴苑琪 刘魏征 范丽华 朱晨辉 段志广* 范代娣* （1.吴苑琪 西北大学化工学院，陕西省西安市 7 10069； 2.刘魏征 西北大学化工学院，陕西省西安市 7 10069） 惠俊峰 教授 摘要 目的：探究稀有人参皂苷对实验性白癜风动物模型的治疗作用。 方法：MTT 法检测人参皂苷 Rh2 和人参皂 苷 Rg3 组合物对鼠黑素瘤细胞增殖活性的影响。采用两种化学脱色法（分别使用氢醌和过氧化氢）制备实验性白 癜风动物模型，经不同剂量 (1.0 g/kg · d 、2.0 g/kg · d) 的人参皂苷干预后，肉眼观察药物治疗实验性白癜风动物模 型的疗效，并拍照记录。 结果：MTT 实验结果表明该组合物对黑素瘤细胞的增殖具有显著的抑制作用。人参皂苷 Rh2 和人参皂苷Rg3 组合物对两种方法建立的白癜风豚鼠模型有良好的治疗作用，能明显地看到经稀有人参皂苷干 预后的白癜风豚鼠模型症状减轻甚至消失。 结论：稀有人参皂苷对实验性白癜风动物模型具有良好的治疗作用， 为临床应用提供了实验依据。 Abstract: Objective : To explore the treatment effects of rare ginsenosides on the experimental vitiligo animal models. Methods : MTT methods were employed to measure the effects of ginsenosides Rh2 and Rg3 on proliferation of murine melanoma cells. The animal models of vitiligo were prepared by two chemical deco loring methods (hydroquinone and hydrogen peroxide), then were separately treated with different doses of ginsenosides( 1.0 g/kg · d, 2.0 g/kg · d). To observe the curative effect of medicinal composition s in the treatment of vitiligo, pictures about experi mental animal models were recorded. Result: The MTT experimental results showed that the combination of ginsenosides Rh2 and Rg3 significantly inhibit the proliferation of melanoma cells. Ginsenosides Rh2 and Rg3 had positive therapeutic effects on experimental animal model of vitiligo established by the two methods. We could obviously observe that the vitiligo symptoms relieved and disappeared after treatment with the rare ginsenosides. Conclusion: Rare ginsenosides has a nice therapeutic effect on experimental animal models of vitiligo and providing an experimental basis for clinical use. Key words: rare ginsenosides; vitiligo; ani