油菜micms恢复基因近等基因系的多态性分析-biopublisher.docVIP

研究论文 A Letter 油菜MI CMS恢复基因近等基因系的构建与近等性分析龙卫华 胡茂龙 高建芹 浦惠明*江苏省农业科学院经济作物研究所国家油菜改良中心南京分中心南京210014 *通讯作者puhuiming@126.com 摘 要 以宁R7为恢复基因源，利用连续回交法构建以T78、T84和ZS9三个优异种质为遗传背景供体的近等基因系候选株系。于成熟期考察并比较候选株系农艺性状。同时采用SSR标记对候选株系进行PCR扩增估算遗传相似系数并进行聚类分析。农艺性状分析(t测验)表明，不同候选株系与其供体的农艺性状具有不同程度的相似性，株高和分支点高度的差异程度较其他性状高。采用75对SSR引物对21个候选株系PCR扩增共得到157个目的片段，发现三个遗传背景候选株系的平均遗传相似系数分别为0.86，0.92和0.98。农艺性状与SSR标记综合分析后，认为wh13、wh59和wh118分别为三个种质的最优近等基因系。综上表明，采用农艺性状比较与分子标记相结合分析近等性是一种有效的近等基因系的检测方法。中选近等基因系可用于恢复基因的定位及克隆。 关键词 恢复基因近等基因系(NIL)近等性分析 Construction of the Near-Isogenic Lines of the MI CMS Restorer Gene in Brassica napus) and Allelic Analysis Long Weihua Hu Maolong Gao Jianqin Pu Huiming* Chen song Zhang Jiefu Qi Cunkou The Industry Crops Institute of Jiangsu Academy of Agriculture Science, Nanjing Branch of National Improvement Center of Rapeseed, Nanjing, 210014 *Corresponding author, puhuiming@126.com Abstract In this research, we employed Ning R7, an elite restorer for MI CMS, as the restorer gene donor to develop restorer gene candidate near-isogenic lines (NILs) by using three elite germplasms, T78, T84 and ZS9, as recurrent parents based on the advanced backcross approach. The characteristics of agronomic traits were phenotyped at the maturity stage and genetic similarity was calculated based on the SSR data. The results showed most agronomic traits of the candidates had quite similar with that of recurrent parents besides the trait of plant height and the height of the initiate branch based on t-test. Further, in order to figure out the similarity of the genetic background, we used 75 SSR markers to generate total 157 SSR loci in the 21 candidate NILs. The average genetic similarity coefficient of the candidate NIL background reached 0.86, 0.92 and 0.98 corresponding to their own recurrent parent, respectively. With the comprehensive assessment, three candidate NILs, wh13、wh59 and wh118 Key words Rapeseed (Brassica napusL), CMS, Restorer gene, Near-Isogenic Lines(NIL), Allelic analysis