乳腺浸润性导管癌组织中PTTG及COX2表达及临床意义.docVIP

乳腺浸润性导管癌组织中PTTG及COX2表达及临床意义 　 作者：夏丹　张庆慧 许复郁　张翠娟　季昌华 【摘要】 　　目的：检测乳腺浸润性导管癌组织中垂体肿瘤转化基因（pituitary tumor-transforming gene, PTTG）和环氧化酶2（cyclooxygenase-2, COX2）的蛋白表达水平并分析与其他临床病理特征的关系。方法:用免疫组织化学Evinson法检测104例乳腺浸润性导管癌组织中PTTG和COX2的蛋白表达水平。结果:104例乳腺浸润性导管癌组织中，有95例检测到PTTG阳性表达，阳性率为91%，其中41例呈低表达（39%），54例呈高表达（52%）。104例乳腺浸润性导管癌组织中，有 70例检测到COX2阳性表达，阳性率为 67%，其中53例呈低表达（51%），17例呈高表达（16%）；PTTG表达与肿瘤大小、组织学分级密切相关，组织学分级越高，PTTG 表达越强，PTTG 表达与年龄、淋巴结转移、ER、PR和HER2状态无关；COX2表达与ER表达、肿瘤大小、组织学分级和淋巴结转移相关，ER（+）组的COX2表达高于 ER（-）组，COX2表达与 PTTG 表达、年龄、PR和HER2 状态无关。结论: PTTG和COX2在乳腺浸润性导管癌组织中的表达可能促进乳腺浸润性导管癌的发生发展,并且与低分化、预后不良有关。COX2可能通过刺激雌激素合成来促进乳腺癌细胞增殖。COX2可能促进乳腺癌侵袭转移。 【关键词】 乳腺肿瘤·垂体肿瘤转化基因·环氧化酶·免疫组织化学 　　Expression of PTTG and COX2 in breast invasive ductal carcinoma and their clinical significance 【ABSTRACT】 Objective: To evaluate the expressions of PTTG and COX2 in breast invasive ductal carcinoma and their clinical significance. Methods: The expressions of PTTG and COX2 were examined in 104 patients with breast invasive duct carcinoma using Evinson techniques. Results: The positive rates of PTTG and COX2 expressions were 91％ and 67％ respectively. There were significant associations between PTTG expression and histological grade and tumor size. There were nosignificant associations between PTTG expression and age, lymph node metastasis, ER, PR, HER2 state. The expressions of COX2 had correlation with ER state, tumor size, histological grade, lymph node metastasis. There were no significant associations between COX2 expressions and PTTG expression and age, PR, HER2 state. Conclusion: The expression of PTTG was possibly involved in the development of breast cancer, and had correlations with low differentiation degree and bad prognosis. COX2 might promote breast cancer proliferation of cells by stimulating estrogen combining. 【KEY WORDS】 Breast neoplasm·PTTG·COX2 　　垂体瘤转化基因（pituitary tumor-transforming gene, PTTG）在许多肿瘤组织中高表达，参与多种肿瘤发生发展全过程，引起国内外学者的关注。环氧化酶（cyclooxygenase-2, COX2）是花生四烯酸代谢成为前列腺素的限速酶，在炎症、肿瘤的发生发展过程中发挥