乳康酊对实验性大鼠乳腺小叶增生模型影响探究.docVIP

乳康酊对实验性大鼠乳腺小叶增生模型影响探究 　 作者：曾宪彪，刘智生，周军，伟刚林 【摘要】 目的研究乳康酊对乳腺小叶增生模型大鼠血清雌、孕激素水平及病变乳腺组织的影响。方法皮下注射雌二醇和黄体酮诱发大鼠乳腺小叶增生，然后在病变乳房处外敷乳康酊,1次/d，共30 d。用药结束后检测血清雌、孕激素水平；显微观察乳腺组织病理变化和乳腺细胞性激素受体（雌激素受体、孕激素受体）。结果受药模型鼠血清雌激素水平和乳腺组织性激素受体阳性腺细胞百分数降低，显微病理见乳腺小叶增生病变减轻。结论乳康酊能够减轻实验性病理模型大鼠乳腺小叶组织增生。 【关键词】 乳康酊； 乳腺小叶增生； 雌激素； 孕激素； 雌激素受体； 孕激素受体 　　Abstract：ObjectiveTo study the effects of Rukangding tincture on the serum level of estrogen, progestin and pathological breast gland tissue of model with experimental breast glands lobular hyperplasia in rat.MethodsExperimental rat lobular hyperplasia of breast glands were induced by hypodermic injection of estradiol and progesterone.And then, Rukangding tincture was applicated on pathological breast surface skin once every day for 30 days. Serum level of estrogen, progestin were examined, pathological changes in the breast gland tissue and sex hormone receptors(estrogen receptor and progestin receptor) in breast gland cell were observed with microscope after the tincture application.ResultsDecrease in the serum level of estrogen and the percent of gland cell with sex hormone receptor were found in the treated rats with Rukangding tincture, and less pathological changes in the gland tissue were also observed with microscope in the treated rats, compared with the control. ConclusionRukangding tincture can reduce lobular hyperplasia of breast glands in rat with experimental pathological model. 　　Key words：Rukangding tincture； Breast glands lobular hyperplasia； Estrogen； Progestin； Estrogen receptors； Progestin receptors 　　乳康酊是广西中医药研究院开发的纯中药制剂，处方由穿山甲、乳香、生南星、莪术、徐长卿、山慈姑等多味中药组成，民间取其水煎稠膏局部外敷，用于妇女乳房肿块等症［1］。本实验给乳腺小叶增生病理模型大鼠乳房局部皮肤敷用乳康酊药液,通过检测敷药后大鼠血清雌、孕激素含量［2,3］，显微观察乳腺细胞性激素受体（雌激素受体、孕激素受体）和乳腺小叶组织形态［4,5］，研究乳康酊对血清雌、孕激素水平及病变乳腺组织的影响。结果见乳康酊能够降低血清雌激素水平和乳腺组织性激素受体阳性腺细胞数，减轻乳腺小叶增生病理变化。现报道如下。 　　1 材料与仪器 　　1.1 受试药物 　　乳康酊是广西中医药研究院研制的中试产品，为棕色粘稠液体，与水容易混合，批号070216，密度1.30，相当生药量10 g生药·ml-1。置阴凉避光处密封保存。实验时取原药和1个水稀释药（浓度：5 g生药·ml-1）使用。 　　1.2 动物 　　雌性未孕Wistar大鼠，25周龄，体质量（180±25）g，清洁级，购自广西医科大学实验动