deletion of dicer in smooth muscle affects voiding pattern and reduces detrusor contractility and neuroeffector transmission删除小礼帽的平滑肌影响排泄,降低逼尿肌收缩和neuroeffector传输模式.pdfVIP

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2017-09-12 发布于上海
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deletion of dicer in smooth muscle affects voiding pattern and reduces detrusor contractility and neuroeffector transmission删除小礼帽的平滑肌影响排泄,降低逼尿肌收缩和neuroeffector传输模式.pdf

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deletion of dicer in smooth muscle affects voiding pattern and reduces detrusor contractility and neuroeffector transmission删除小礼帽的平滑肌影响排泄,降低逼尿肌收缩和neuroeffector传输模式

Deletion of Dicer in Smooth Muscle Affects Voiding Pattern and Reduces Detrusor Contractility and Neuroeffector Transmission 1 1 1 2 ¨ 1. Mardjaneh Karbalaei Sadegh , Mari Ekman , Catarina Rippe , Bengt Uvelius , Karl Sward , 1 . Sebastian Albinsson * 1 Department of Experimental Medical Science, Biomedical Centre, Lund University, Lund, Sweden, 2 Department of Urology, Clinical Sciences, Lund University, Lund, Sweden Abstract MicroRNAs have emerged as important regulators of smooth muscle phenotype and may play important roles in pathogenesis of various smooth muscle related disease states. The aim of this study was to investigate the role of miRNAs for urinary bladder function. We used an inducible and smooth muscle specific Dicer knockout (KO) mouse which resulted in significantly reduced levels of miRNAs, including miR-145, miR-143, miR-22, miR125b-5p and miR-27a, from detrusor preparations without mucosa. Deletion of Dicer resulted in a disturbed micturition pattern in vivo and reduced depolarization-induced pressure development in the isolated detrusor. Furthermore, electrical field stimulation revealed a decreased cholinergic but maintained purinergic component of neurogenic activation in Dicer KO bladder strips. The ultrastructure of detrusor smooth muscle cells was well maintained, and the density of nerve terminals was similar. Western blotting demonstrated reduced contents of calponin and desmin. Smooth muscle a-actin, SM22a and myocardin were unchanged. A