deletion of running-induced hippocampal neurogenesis by irradiation prevents development of an anxious phenotype in mice删除running-induced海马神经发生辐照阻止发展的焦虑的小鼠的表型.pdfVIP

Deletion of Running-Induced Hippocampal Neurogenesis by Irradiation Prevents Development of an Anxious Phenotype in Mice 1 . 1. 2 2 3 Johannes Fuss * , Nada M. B. Ben Abdallah , Frank W. Hensley , Klaus-Josef Weber , Rainer Hellweg , Peter Gass1 1 Department of Psychiatry and Psychotherapy, Central Institute of Mental Health Mannheim (ZI), University of Heidelberg, Mannheim, Germany, 2 Department of ´ Radiooncology, University of Heidelberg, Heidelberg, Germany, 3 Department of Psychiatry, University of Medicine of Berlin, Campus Charite Mitte, Berlin, Germany Abstract Recent evidence postulates a role of hippocampal neurogenesis in anxiety behavior. Here we report that elevated levels of neurogenesis elicit increased anxiety in rodents. Mice performing voluntary wheel running displayed both highly elevated levels of neurogenesis and increased anxiety in three different anxiety-like paradigms: the open field, elevated O-maze, and dark-light box. Reducing neurogenesis by focalized irradiation of the hippocampus abolished this exercise-induced increase of anxiety, suggesting a direct implication of hippocampal neurogenesis in this phenotype. On the other hand, irradiated mice explored less frequently the lit compartment of the dark-light box test irrespective of wheel running, suggesting that irradiation per se induced anxiety as well. Thus, our data suggest that intermediate levels of neurogenesis are related to the lowest levels of anxiety. Moreover, using c-Fos immunocytochemistry as cellular activity marker, we observed significantly different induction patterns