deep sequencing of myc dna-binding sites in burkitt lymphoma深度测序myc dna结合网站的伯基特淋巴瘤.pdfVIP

Deep Sequencing of MYC DNA-Binding Sites in Burkitt Lymphoma 1 2 1 3 ¨ 4 1 Volkhard Seitz , Peter Butzhammer , Burkhard Hirsch , Jochen Hecht , Ines Gutgemann , Anke Ehlers , 1 1 1 1 ¨ 5 Dido Lenze , Elisabeth Oker , Anke Sommerfeld , Edda von der Wall , Christoph Konig , Christian 6 2 1 Zinser , Rainer Spang , Michael Hummel * ´ 1 Institute of Pathology, Charite - University Medicine, Campus Benjamin Franklin, Berlin, Germany, 2 Institute for Functional Genomics, University of Regensburg, ´ Regensburg, Germany, 3 Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charite - University Medicine, Berlin, Germany, 4 Department of Pathology, University Hospital of Bonn, Bonn, Germany, 5 imaGenes GmbH, Berlin, Germany, 6 Genomatix Software GmbH, Munich, Germany Abstract Background: MYC is a key transcription factor involved in central cellular processes such as regulation of the cell cycle, histone acetylation and ribosomal biogenesis. It is overexpressed in the majority of human tumors including aggressive B- cell lymphoma. Especially Burkitt lymphoma (BL) is a highlight example for MYC overexpression due to a chromosomal translocation involving the c-MYC gene. However, no genome-wide analysis of MYC-binding sites by chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) has been conducted in BL