copi is required for enterovirus 71 replicationcopi肠病毒71需要复制.pdfVIP

COPI Is Required for Enterovirus 71 Replication Jianmin Wang, Zhiqiang Wu, Qi Jin* State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China Abstract Enterovirus 71 (EV71), a member of the Picornaviridae family, is found in Asian countries where it causes a wide range of human diseases. No effective therapy is available for the treatment of these infections. Picornaviruses undergo RNA replication in association with membranes of infected cells. COPI and COPII have been shown to be involved in the formation of picornavirus-induced vesicles. Replication of several picornaviruses, including poliovirus and Echovirus 11 (EV11), is dependent on COPI or COPII. Here, we report that COPI, but not COPII, is required for EV71 replication. Replication of EV71 was inhibited by brefeldin A and golgicide A, inhibitors of COPI activity. Furthermore, we found EV71 2C protein interacted with COPI subunits by co-immunoprecipitation and GST pull-down assay, indicating that COPI coatomer might be directed to the viral replication complex through viral 2C protein. Additionally, because the pathway is conserved among different species of enteroviruses, it may represent a novel target for antiviral therapies. Citation: Wang J, Wu Z, Jin Q (2012) COPI Is Required for Enterovirus 71 Replication. PLoS ONE 7(5): e38035. doi:10.1371/journal.pone.0038035 ´ ´ Editor: Luis Menendez-Arias, Centro de Biologıa Molecular Severo Ochoa (CSIC-UAM), Spain Received January 4, 2012; Accepted April