有哪些信誉好的足球投注网站coronin is a component of the endocytic collar of hyphae of neurospora crassa and is necessary for normal growth and morphogenesiscoronin是一个组件的内吞作用的衣领粗糙脉孢菌菌丝和正常生长和形态发生是必要的.pdfVIP
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coronin is a component of the endocytic collar of hyphae of neurospora crassa and is necessary for normal growth and morphogenesiscoronin是一个组件的内吞作用的衣领粗糙脉孢菌菌丝和正常生长和形态发生是必要的
Coronin Is a Component of the Endocytic Collar of Hyphae of Neurospora crassa and Is Necessary for Normal Growth and Morphogenesis 1 1 2 Ramon O. Echauri-Espinosa , Olga A. Callejas-Negrete , Robert W. Roberson , Salomon Bartnicki- ´ 1 ˜ ´ 1 Garcıa , Rosa R. Mourino-Perez * ´ ´ ´ ´ ´ 1 Departamento de Microbiologıa, Centro de Investigacion Cientıfica y de Educacion Superior de Ensenada (CICESE), Ensenada, Baja California, Mexico, 2 School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America Abstract Coronin plays a major role in the organization and dynamics of actin in yeast. To investigate the role of coronin in a filamentous fungus (Neurospora crassa), we examined its subcellular localization using fluorescent proteins and the ¨ phenotypic consequences of coronin gene (crn-1) deletion in hyphal morphogenesis, Spitzenkorper behavior and endocytosis. Coronin-GFP was localized in patches, forming a subapical collar near the hyphal apex; significantly, it was absent from the apex. The subapical patches of coronin colocalized with fimbrin, Arp2/3 complex, and actin, altogether comprising the endocytic collar. Deletion of crn-1 resulted in reduced hyphal growth rates, distorted hyphal morphology, uneven wall thickness, and delayed establishment of polarity during germination; it also affected growth directional
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