common gamma chain cytokines promote rapid in vitro expansion of allo-specific human cd8+ suppressor t cells常见的γ链细胞因子促进体外迅速扩张allo-specific人类的cd8 + t细胞抑制.pdfVIP

Common Gamma Chain Cytokines Promote Rapid In Vitro Expansion of Allo-Specific Human CD8+ Suppressor T Cells 1,2 1 1 1 1 1 Yuming Yu , Jennifer R. Zitzner , Josetta Houlihan , Nancy Herrera , Luting Xu , Joshua Miller , 1 1 1,3 James M. Mathew , Anat R. Tambur , Xunrong Luo * 1 Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America, 2 Department of Organ Transplantation, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China, 3 Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America Abstract + + 2 Human CD8 regulatory T cells, particularly the CD8 CD28 T suppressor cells, have emerged as an important modulator of alloimmunity. Understanding the conditions under which these cells are induced and/or expanded would greatly facilitate their application in future clinical trials. In the current study, we develop a novel strategy that combines common gamma chain (cc) cytokines IL-2, IL-7 and IL-15 and donor antigen presenting cells (APCs) to stimulate full HLA-mismatched allogeneic human CD8+ + 2 T cells which results in significant expansions of donor-specific CD8 CD28 T suppressor cells in vitro. The expanded CD8+CD282 T cells exhibit increased expressions of CTLA-4, FoxP3, and CD25, while down-regulate expressions of CD56, CD57, CD127,