α-calcium calmodulin kinase ii modulates the temporal structure of hippocampal bursting patternsα-calcium钙调蛋白激酶ⅱ调节海马破裂的时间结构模式.pdfVIP

a-Calcium Calmodulin Kinase II Modulates the Temporal Structure of Hippocampal Bursting Patterns 1,2,3 4 1,5 1 Jeiwon Cho , Rushi Bhatt , Ype Elgersma , Alcino J. Silva * 1 Departments of Neurobiology, Psychiatry, and Psychology, Brain Research Institute, University of California Los Angeles, Los Angeles, California, United States of America, 2 Center for Neural Science, Korea Institute of Science and Technology, Seongbuk-gu, Seoul, Korea, 3 Department of Neuroscience, University of Science and Technology, Seoul, Korea, 4 Yahoo! SDC, Embassy Golf Links Business Park, Bangalore, India, 5 Department of Neuroscience, Erasmus MC, Rotterdam, The Netherlands Abstract The alpha calcium calmodulin kinase II (a-CaMKII) is known to play a key role in CA1/CA3 synaptic plasticity, hippocampal place cell stability and spatial learning. Additionally, there is evidence from hippocampal electrophysiological slice studies that this kinase has a role in regulating ion channels that control neuronal excitability. Here, we report in vivo single unit studies, with a-CaMKII mutant mice, in which threonine 305 was replaced with an aspartate (a-CaMKIIT305D mutants), that indicate that this kinase modulates spike patterns in hippocampal pyramidal neurons. Previous studies showed that a- CaMKIIT305D mutants have abnormalities in both hippocampal LTP and hippocampal-dependent learning. We found that besides decreased place cell stability, which could be caused by their LTP impairments, the hippocampal CA1 spike patterns of a-CaMKIIT305D mutants were profoundly abnormal. Although overall firing rate, and overall burst frequency were not significantly altered in these mutants, inter-burst intervals, mean number of intra-burs