有哪些信誉好的足球投注网站wide-field multi-parameter flim long-term minimal invasive observation of proteins in living cells大视场多参数这部电影长期微创观察活细胞的蛋白质.pdfVIP
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wide-field multi-parameter flim long-term minimal invasive observation of proteins in living cells大视场多参数这部电影长期微创观察活细胞的蛋白质
Wide-Field Multi-Parameter FLIM: Long-Term Minimal Invasive Observation of Proteins in Living Cells 1¤ 2 1 3 1,4 ¨ 3 Marco Vitali , Fernando Picazo , Yury Prokazov , Alessandro Duci , Evgeny Turbin , Christian Gotze , 2 5 6 1 Juan Llopis , Roland Hartig , Antonie J. W. G. Visser , Werner Zuschratter * 1 Leibniz Institute for Neurobiology, Magdeburg, Germany, 2 University of Castilla-La Mancha, Albacete, Spain, 3 Arivis Multiple Image Tools GmbH, Rostock, Germany, 4 Russian Research Center Kurchatov Institute, Moscow, Russia, 5 Otto-von-Guericke University, Magdeburg, Germany, 6 Wageningen University, Wageningen, The Netherlands Abstract Time-domain Fluorescence Lifetime Imaging Microscopy (FLIM) is a remarkable tool to monitor the dynamics of fluorophore-tagged protein domains inside living cells. We propose a Wide-Field Multi-Parameter FLIM method (WFMP- FLIM) aimed to monitor continuously living cells under minimum light intensity at a given illumination energy dose. A powerful data analysis technique applied to the WFMP-FLIM data sets allows to optimize the estimation accuracy of physical parameters at very low fluorescence signal levels approaching the lower bound theoretical limit. We demonstrate the efficiency of WFMP-FLIM by presenting two independent and relevant long-term experiments in cell biology: 1) FRET analysis of simultaneously recorded donor and acceptor fluorescence in living HeLa cells and 2) tracking of mitochondrial transport comb
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