the bone-forming effects of hif-1α-transduced bmscs promote osseointegration with dental implant in canine mandible的成骨效应hif-1α-transduced bmsc促进与牙科植体在犬下颌骨的骨整合.pdfVIP

The Bone-Forming Effects of HIF-1a-Transduced BMSCs Promote Osseointegration with Dental Implant in Canine Mandible 1,2 2 3 1 1 1 2 Duohong Zou , Jiacai He , Kai Zhang , JieWen Dai , Wenjie Zhang , Shaoyi Wang , Jian Zhou , 4 1 . 1 . Yuanliang Huang , Zhiyuan Zhang * , Xinquan Jiang * 1 Ninth People’s Hospital Affiliated with Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China, 2 Department of Oral and Maxillofacial Surgery, School of Stomatology, Stomatological Hospital, Anhui Medical University, Hefei, China, 3 Department of Stomatology, First Affiliated Hospital of Bengbu Medical College, Anhui, China, 4 Department of Stomatology, Shanghai East Hospital Affiliated with Tongji University, Shanghai, China Abstract The presence of insufficient bone volume remains a major clinical problem for dental implant placement to restore the oral function. Gene-transduced stem cells provide a promising approach for inducing bone regeneration and enhancing osseointegration in dental implants with tissue engineering technology. Our previous studies have demonstrated that the hypoxia-inducible factor-1a (HIF-1a) promotes osteogenesis in rat bone mesenchymal stem cells (BMSCs). In this study, the function of HIF-1a was validated for the first time in a preclinical large animal canine model in term of its ability to promote new bone formation in defects around implants as well as the osseointegration between tissue-engineered bone and dental implants. A lentiviral vector was constructed with the constitutively active form of HIF-1a (cHIF). The ectopic bone fo