studies in the use of magnetic microspheres for immunoaffinity extraction of paralytic shellfish poisoning toxins from shellfish研究磁性微球的使用immunoaffinity提取从贝类麻痹性贝类中毒的毒素.pdfVIP

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studies in the use of magnetic microspheres for immunoaffinity extraction of paralytic shellfish poisoning toxins from shellfish研究磁性微球的使用immunoaffinity提取从贝类麻痹性贝类中毒的毒素.pdf

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studies in the use of magnetic microspheres for immunoaffinity extraction of paralytic shellfish poisoning toxins from shellfish研究磁性微球的使用immunoaffinity提取从贝类麻痹性贝类中毒的毒素

Toxins 2011, 3, 1-16; doi:10.3390/toxins3010001 OPEN ACCESS toxins ISSN 2072-6651 /journal/toxins Article Studies in the Use of Magnetic Microspheres for Immunoaffinity Extraction of Paralytic Shellfish Poisoning Toxins from Shellfish Raymond Devlin 1, Katrina Campbell 1, Kentaro Kawatsu 2 and Christopher Elliott 1,* 1 Institute of Agri-Food and Land Use, School of Biological Sciences, Queen’s University Belfast, Stranmillis Road, Belfast, BT9 5AG, Northern Ireland, UK; E-Mails: rdevlin04@qub.ac.uk (R.D.); Katrina.Campbell@qub.ac.uk (K.C.) 2 Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka, Japan; E-Mail: kawatu@iph.pref.osaka.jp * Author to whom correspondence should be addressed; E-Mail: Chris.Elliott@qub.ac.uk; Tel.: +44-28-9097-6549; Fax: +44-28-9097-6513. Received: 19 November 2010; in revisedform: 10 December 2010 / Accepted: 14 December 2010 / Published: 4 January 2011 Abstract: Paralytic shellfish poisoning (PSP) is a potentially fatal human health condition caused by the consumption of shellfish containing high levels of PSP toxins. Toxin extraction from shellfish and from algal cultures for use as standards and analysis by alternative analytical monitoring methods to the mouse bioassay is extensive and laborious. This study investigated whether a selected MAb antibody could be coupled to a novel form of magnetic microsphere (hollow glass magnetic microspheres, brand name Ferros