桂枝茯苓胶囊对肾上腺素诱导大鼠试验性肠系膜微循环障碍的改善研究.PDFVIP

·8 · 现代药物与临床 Drugs Clinic 第 30 卷 第 1 期 2015 年 1 月 桂枝茯苓胶囊对肾上腺素诱导大鼠实验性肠系膜微循环障碍的改善研究 孙 兰 1, 2，李家春 1, 2，周 军 1, 2，王振中 1, 2*，萧 伟 1, 2* 1. 江苏康缘药业股份有限公司，江苏 连云港 222001 2. 中药制药过程新技术国家重点实验室，江苏 连云港 222001 摘 要：目的 研究桂枝茯苓胶囊对大鼠实验性肠系膜微循环障碍的影响。方法 将 75 只 SD 雌性大鼠随机分为 5 组，分 别为模型组、盐酸消旋山莨菪碱注射液（0.50 mg/只，im 给药）组以及桂枝茯苓胶囊低、中、高剂量（0.14、0.27、0.54 g/kg， ig 给药）组，1 次/d ，连续 7 d 。末次给药后30 min 开腹找出肠系膜，观察滴加肾上腺素后选定区域的微动脉管径、微静脉 管径、微动脉流速、微静脉流速及局部微循环血流恢复时间。结果 局部区域滴加 1 ∶100 盐酸肾上腺素生理盐水溶液 20 μL/ 只后，观察区出现了典型的微循环障碍的表现。桂枝茯苓胶囊对微动脉管径、微静脉管径，微动脉流速，微静脉流速及局部 微循环血流恢复时间均有显著的改善作用。结论 桂枝茯苓胶囊具有改善微循环障碍作用。 关键词：桂枝茯苓胶囊；实验性肠系膜微循环障碍；微动脉管径；改善作用 中图分类号：R285.5 文献标志码：A 文章编号：1674 - 5515(2015)01 - 0008 - 05 DOI: 10.7501/j.issn.1674-5515.2015.01.002 Improvement of Guizhi Fuling Capsules on epinephrine induced experimental rat mesenteric microcirculatory disturbance SUN Lan1, 2, LI Jia-chun1, 2, ZHOU Jun1, 2, WANG Zhen-zhong1, 2, XIAO Wei1, 2 1. Kanion Pharmaceutical Co., Ltd, Lianyungang 222001, China 2. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China Abstract: Objective To study the effects of microcirculatory disturbance with Guizhi Fuling Capsules in rats. Methods Seventy-five SD female rats were randomly divided into model group, Raceanisodamine Hydrochloride Injection (0.50 mg each,, im administration) group, Guizhi Fuling Capsules low-, mid-, and high-dose (0.14, 0.27, and 0.54 g/kg, ig administration) groups. At the same time mice were given drugs 1 once daily for 7 d. Thirty minutes after intragastric administration on the 7th day, added epinephrine and observed the micro artery diameter, microvein diameter, micro artery flow velocity, micro vein flow velocity, and recovery time of blood flow. Results 20 μL of Adrenaline Saline Solution (1 ∶100) was added into in local area and appeared typically microcirculatory disturbance. Guizhi F