analysis of transcriptional regulatory pathways of photoreceptor genes by expression profiling of the otx2-deficient retina分析感光基因的转录调控通路的表达分析otx2-deficient视网膜.pdfVIP

Analysis of Transcriptional Regulatory Pathways of Photoreceptor Genes by Expression Profiling of the Otx2- Deficient Retina 1,2,3. 1. 1,4,5. 1 1,2 Yoshihiro Omori , Kimiko Katoh , Shigeru Sato , Yuki Muranishi , Taro Chaya , Akishi 1,2 6 4,5 1,2 Onishi , Takashi Minami , Takashi Fujikado , Takahisa Furukawa * 1 Department of Developmental Biology, Osaka Bioscience Institute, Osaka, Japan, 2 JST, CREST, Osaka, Japan, 3 PRESTO, Suita, Osaka, Japan, 4 Department of Ophthalmology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan, 5 Department of Visual Science, Osaka University Graduate School of Medicine, Suita, Osaka, Japan, 6 The Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan Abstract In the vertebrate retina, the Otx2 transcription factor plays a crucial role in the cell fate determination of both rod and cone photoreceptors. We previously reported that Otx2 conditional knockout (CKO) mice exhibited a total absence of rods and cones in the retina due to their cell fate conversion to amacrine-like cells. In order to investigate the entire transcriptome of the Otx2 CKO retina, we compared expression profile of Otx2 CKO and wild-type retinas at P1 and P12 using microarray. We observed that expression of 101- and 1049-probe sets significantly decreased in the Otx2 CKO retina at P1 and P12, respectively, whereas, expression of 3- and 4149-probe sets increased at P1 and P12, respectively. We found that expression of genes encoding transcription factors involved in photoreceptor development, includ