analysis of the transcriptomes downstream of eyeless and the hedgehog, decapentaplegic and notch signaling pathways in drosophila melanogaster下游转录组的分析没有眼睛的刺猬,decapentaplegic notch信号通路在黑腹果蝇.pdfVIP

Analysis of the Transcriptomes Downstream of Eyeless and the Hedgehog, Decapentaplegic and Notch Signaling Pathways in Drosophila melanogaster 1 1 2 2 1 Landry E. Nfonsam , Carlos Cano , Joann Mudge , Faye D. Schilkey , Jennifer Curtiss * 1 Department of Biology, New Mexico State University, Las Cruces, New Mexico, United States of America, 2 National Center for Genome Resources, Santa Fe, New Mexico, United States of America Abstract Tissue-specific transcription factors are thought to cooperate with signaling pathways to promote patterned tissue specification, in part by co-regulating transcription. The Drosophila melanogaster Pax6 homolog Eyeless forms a complex, incompletely understood regulatory network with the Hedgehog, Decapentaplegic and Notch signaling pathways to control eye-specific gene expression. We report a combinatorial approach, including mRNAseq and microarray analyses, to identify targets co-regulated by Eyeless and Hedgehog, Decapentaplegic or Notch. Multiple analyses suggest that the transcriptomes resulting from co-misexpression of Eyeless+signaling factors provide a more complete picture of eye development compared to previous efforts involving Eyeless alone: (1) Principal components analysis and two-way hierarchical clustering revealed that the Eyeless+signaling factor transcriptomes are closer to the eye control transcriptome than when Eyeless is misexpressed alone; (2) more genes are upregulated at least three-fold in response to Eyeless+signaling factors compared to Eyeless alone; (3) based on gene ontology analysis, the genes upregulated in respon