analysis of the plant bos1 mutant highlights necrosis as an efficient defence mechanism during d. dadantiiarabidospis thaliana interaction植物bos1突变体的分析突出了坏死作为一个有效的防御机制在d . dadantiiarabidospis芥交互.pdfVIP

Analysis of the Plant bos1 Mutant Highlights Necrosis as an Efficient Defence Mechanism during D. dadantii/ Arabidospis thaliana Interaction 1,2,3. ´ 1,2,3. 1,2,3 ˆ 1,2,3 Yvan Kraepiel , Jacques Pedron , Oriane Patrit , Elizabeth Simond-Cote , Victor 1,2,3 ´ ´ 1,2,3 Hermand , Frederique Van Gijsegem * ` ` 1 INRA, UMR217, Interactions Plantes-Pathogenes, Paris, France, 2 UPMC Univ Paris 06, UMR217, Interactions Plantes-Pathogenes, Paris, France, 3 AgroParisTech, UMR217, ` Interactions Plantes-Pathogenes, Paris, France Abstract Dickeya dadantii is a broad host range phytopathogenic bacterium provoking soft rot disease on many plants including Arabidopsis. We showed that, after D. dadantii infection, the expression of the Arabidopsis BOS1 gene was specifically induced by the production of the bacterial PelB/C pectinases able to degrade pectin. This prompted us to analyze the interaction between the bos1 mutant and D. dadantii. The phenotype of the infected bos1 mutant is complex. Indeed, maceration symptoms occurred more rapidly in the bos1 mutant than in the wild type parent but at a later stage of infection, a necrosis developed around the inoculation site that provoked a halt in the progression of the maceration. This necrosis became systemic and spread throughout the whole plant, a phenotype reminiscent of that observed in some lesion mimic mutants. In accordan