an electrochemical method to detect gamma glutamyl transpeptidase一种电化学方法检测γ谷酰基转肽酶.pdfVIP

Int. J. Mol. Sci. 2012, 13, 2801-2809; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article An Electrochemical Method to Detect Gamma Glutamyl Transpeptidase Guifang Chen 1, Shengfa Ni 2, Sha Zhu 2, Jinghua Yang 3 and Yongmei Yin 2,* 1 Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China; E-Mail: gfchen@ 2 Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; E-Mails: inzo@163.com (S.N.); nanyizhusha@126.com (S.Z.) 3 Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China; E-Mail: josephinenju@ * Author to whom correspondence should be addressed; E-Mail: YMYin2011@; Tel.: +86-25 Fax: +86-25 Received: 31 December 2011; in revised form: 3 February 2012 / Accepted: 27 February 2012 / Published: 2 March 2012 Abstract: Gamma glutamyl transpeptidase (GGT) is a transferase, which is of great importance in sustaining intracellular cysteine and glutathione levels. The abnormal expression of GGT is significantly associated with features of many metabolic syndromes (e.g., hepatocellular carcinoma). Therefore, it is essential to develop methods to detect GGT so as to monitor the physiological or pathological phenomena related to this specie