analysis of phenacylester derivatives of fatty acids from human skin surface sebum by reversed-phase hplc chromatographic mobility as a function of physico-chemical propertiesphenacylester衍生物脂肪酸的分析从人体皮肤表面油脂反相高效液相色谱色谱流动的物理化学性质.pdfVIP

Int. J. Mol. Sci. 2007, 8, 1111-1124 International Journal of Molecular Sciences ISSN 1422-0067 © 2007 by MDPI /ijms Full Research Paper Analysis of Phenacylester Derivatives of Fatty Acids from Human Skin Surface Sebum by Reversed-Phase HPLC: Chromatographic Mobility as a Function of Physico-Chemical Properties Juliana Bodoprost and Helmut Rosemeyer * Organic Materials Chemistry - Bioorganic Chemistry, Institute of Chemistry, University of Osnabrück, Barbarastr. 7, D-49069 Osnabrück, Germany * Author to whom correspondence should be addressed; E-mail: Helmut.Rosemeyer@uos.de Received: 28 August 2007; in revised form: 8 November 2007 / Accepted: 12 November 2007 Published: 21 November 2007 Abstract: A set of 13 fatty acids was transformed into their phenacyl esters by reaction with phenacyl bromide in acetonitrile using 18-crown-6 as phase-transfer catalyst. Conditions for the RP-18 HPL chromatographic separation of most of the esters has been worked out. Using this standard the fatty acid spectra from skin surface sebum lipids of 17 test persons was taken after microwave-assisted hydrolysis, neutralization and extraction with n-hexane. Quantitative evaluation of the chromatograms exhibits that oleic acid predominates in the sebum of all test persons. In the second part of the work the chromatographic mobility (RE values) of fatty acid phenacyl esters is correlated with calculated physico-chemical parameters of the correspondi