analysis of intracellular state based on controlled 3d nanostructures mediated surface enhanced raman scattering分析基于控制三维纳米结构介导的细胞内状态表面增强拉曼散射.pdfVIP

Analysis of Intracellular State Based on Controlled 3D Nanostructures Mediated Surface Enhanced Raman Scattering 1 2 1,2 1,2 Waleed Ahmed El-Said , Tae-Hyung Kim , Hyuncheol Kim , Jeong-Woo Choi * 1 Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, Republic of Korea, 2 Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, Republic of Korea Abstract Near-infrared surface-enhanced Raman spectroscopy (SERS) is a powerful technique for analyzing the chemical composition within a single living cell at unprecedented resolution. However, current SERS methods employing uncontrollable colloidal metal particles or non-uniformly distributed metal particles on a substrate as SERS-active sites show relatively low reliability and reproducibility. Here, we report a highly-ordered SERS-active surface that is provided by a gold nano-dots array based on thermal evaporation of gold onto an ITO surface through a nanoporous alumina mask. This new combined technique showed a broader distribution of hot spots and a higher signal-to-noise ratio than current SERS techniques due to the highly reproducible and uniform geometrical structures over a large area. This SERS-active surface was applied as cell culture system to study living cells in situ within their culture environment without any external preparation processes. We applied this newly developed method to cell-based research to differentiate cell lines, cells at different cell cycle stages, and live/dead cells. The enhanced Raman signals achieved from each cell, which represent the changes in biochemical compositions, enabled differentiation of each state and