有哪些信誉好的足球投注网站active site mutations change the cleavage specificity of neprilysin活性位点突变改变neprilysin的乳沟特异性.pdfVIP
- 1、有哪些信誉好的足球投注网站（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
active site mutations change the cleavage specificity of neprilysin活性位点突变改变neprilysin的乳沟特异性
Active Site Mutations Change the Cleavage Specificity of
Neprilysin
1 1 1 2 1
Travis Sexton , Lisa J. Hitchcook , David W. Rodgers , Luke H. Bradley , Louis B. Hersh *
1 Department of Molecular and Cellular Biochemistry, The Center for Structural Biology, University of Kentucky, Lexington, Kentucky, United States of America,
2 Department of Anatomy and Neurobiology, University of Kentucky, Lexington, Kentucky, United States of America
Abstract
Neprilysin (NEP), a member of the M13 subgroup of the zinc-dependent endopeptidase family is a membrane bound
peptidase capable of cleaving a variety of physiological peptides. We have generated a series of neprilysin variants
containing mutations at either one of two active site residues, Phe563 and Ser546. Among the mutants studied in detail we
observed changes in their activity towards leucine5-enkephalin, insulin B chain, and amyloid b1–40. For example, NEPF563I
5 S546E
displayed an increase in preference towards cleaving leucine -enkephalin relative to insulin B chain, while mutant NEP
was less discriminating than neprilysin. Mutants NEPF563L and NEPS546E exhibit different cleavage site preferences than
neprilysin with insulin B chain and amyloid ß1–40 as substrates. These data indicate that it is possible to alter the cleavage
site specificity of neprilysin opening the way for the development of substrate specific or substrate exclusive forms of the
enzyme with enhanced therapeutic potential.
Citation: Sexton T, Hitchcook LJ, Rodgers DW, Bradley LH, Hersh LB (2012) Active Site Mutations Cha
您可能关注的文档
- a visual analytics approach for extracting spatio-temporal urban mobility information from mobile network traffic视觉分析方法提取时空城市流动信息从移动网络流量.pdf
- a versatile overexpression strategy in the pathogenic yeast candida albicans identification of regulators of morphogenesis and fitness多功能超表达策略在监管机构的白色念珠菌致病酵母鉴定的形态发生和健身.pdf
- a weakened transcriptional enhancer yields variegated gene expression一个被削弱了的转录增强收益组合基因表达.pdf
- a voltage-gated h+ channel underlying ph homeostasis in calcifying coccolithophores几种h +通道底层ph钙化颗石藻的动态平衡.pdf
- a viral ubiquitin ligase has substrate preferential sumo targeted ubiquitin ligase activity that counteracts intrinsic antiviral defence病毒泛素连接酶具有底物优惠相扑有针对性的泛素连接酶活动抵消内在的抗病毒防御.pdf
- a viral satellite rna induces yellow symptoms on tobacco by targeting a gene involved in chlorophyll biosynthesis using the rna silencing machinery卫星rna病毒诱导黄色烟草出现症状通过瞄准一个基因参与叶绿素生物合成使用rna沉默机制.pdf
- a walk into the luxr regulators of actinobacteria phylogenomic distribution and functional diversity走进luxr监管者的放线菌phylogenomic分布和功能的多样性.pdf
- a whole-genome snp association study of nci60 cell line panel indicates a role of ca2+ signaling in selenium resistance全基因组snp协会nci60细胞系的研究小组表明硒抗钙离子信号的作用.pdf
- a wireless multi-channel recording system for freely behaving mice and rats无线多通道记录系统对小鼠和大鼠的行为.pdf
- a widespread distribution of genomic cemyod binding sites revealed and cross validated by chip-chip and chip-seq techniques广泛分布的基因组cemyod结合位点chip-chip和chip-seq技术揭示和交叉验证.pdf
- active transport of bile acids decreases mucin 2 in neonatal ileum implications for development of necrotizing enterocolitis主动运输的胆汁酸减少粘蛋白2在新生儿坏死性小肠结肠炎回肠影响发展.pdf
- activin in the brain modulates anxiety-related behavior and adult neurogenesis苯丙酸诺龙在大脑中调节焦虑性行为和成年神经发生.pdf
- active suppression of early immune response in tobacco by the human pathogen salmonella typhimurium早期免疫反应的活性抑制烟草的人类病原体鼠伤寒沙门氏菌.pdf
- active transport of the ubiquitin ligase mid1 along the microtubules is regulated by protein phosphatase 2a主动运输的泛素连接酶mid1沿着微管是由蛋白磷酸酶2 a.pdf
- active spatial perception in the vibrissa scanning sensorimotor system活跃的空间知觉触须扫描感觉运动系统.pdf
- activity of the upstream component of tandem tertsurvivin promoters depends on features of the downstream component活动的上游组件串联tertsurvivin推动者取决于下游组件的特性.pdf
- active listening for spatial orientation in a complex auditory scene积极倾听的空间定位在一个复杂的听觉场景.pdf
- active versus passive training of a complex bimanual task is prescriptive proprioceptive information sufficient for inducing motor learning一个复杂的用双手的主动与被动训练任务是说明性的本体感受的信息满足诱导运动学习.pdf
- active-site inhibitors of mtor target rapamycin-resistant outputs of mtorc1 and mtorc2的活性位点抑制剂mtor mtorc1和mtorc2 rapamycin-resistant输出目标.pdf
- activity predicts male reproductive success in a polygynous lizard在一夫多妻制蜥蜴活动预测男性生殖成功.pdf
文档评论（0）