有哪些信誉好的足球投注网站activation of the dormant secondary metabolite production by introducing gentamicin-resistance in a marine-derived penicillium purpurogenum g59激活休眠的次生代谢物生产中通过引入gentamicin-resistance marine-derived青霉菌purpurogenum g59.pdfVIP
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activation of the dormant secondary metabolite production by introducing gentamicin-resistance in a marine-derived penicillium purpurogenum g59激活休眠的次生代谢物生产中通过引入gentamicin-resistance marine-derived青霉菌purpurogenum g59
Mar. Drugs 2012, 10, 559-582; doi:10.3390/m
OPEN ACCESS
Marine Drugs
ISSN 1660-3397
/journal/marinedrugs
Article
Activation of the Dormant Secondary Metabolite Production by
Introducing Gentamicin-Resistance in a Marine-Derived
Penicillium purpurogenum G59
Yun-Jing Chai, Cheng-Bin Cui *, Chang-Wei Li, Chang-Jing Wu, Cong-Kui Tian and Wei Hua
Beijing Institute of Pharmacology and Toxicology, 27 Tai-Ping Road, Haidian District,
Beijing 100850, China; E-Mails: chaiyunjing@ (Y.-J.C.); sdrlcw@ (C.-W.L.);
wucj2009@163.com (C.-J.W.); tiantian200102@163.com (C.-K.T.); huawei1980@ (W.H.)
* Author to whom correspondence should be addressed; E-Mail: cuicb@ or
cuicb@126.com; Tel./Fax: +86-10-6821-1656.
Received: 22 November 2011; in revised form: 13 February 2012 / Accepted: 21 February 2012 /
Published: 2 March 2012
Abstract: A new approach to activate silent gene clusters for dormant secondary
metabolite production has been developed by introducing gentamicin-resistance to an
originally inactive, marine-derived fungal strain Penicillium purpurogenum G59. Upon
treatment of the G59 spores with a high concentration of gentamicin in aqueous DMSO, a
total of 181 mutants were obtained by single colony isolation. In contrast to the strain G59,
the EtOAc extracts of nine mutant cultures showed inhibitory effects on K562 cells,
indicating that the nine mutants had acquired capability to produce antitumor metabolites.
This was evidenced by TLC and HPLC analysis of EtOAc extracts
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