有哪些信誉好的足球投注网站activation of pluripotency genes in human fibroblast cells by a novel mrna based approach人类成纤维细胞多能性基因的激活了一种新颖的基于信使rna的方法.pdfVIP
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activation of pluripotency genes in human fibroblast cells by a novel mrna based approach人类成纤维细胞多能性基因的激活了一种新颖的基于信使rna的方法
Activation of Pluripotency Genes in Human Fibroblast
Cells by a Novel mRNA Based Approach
1,2. 1. 1 1 2 1
Jordan R. Plews , JianLiang Li , Mark Jones , Harry D. Moore , Chris Mason , Peter W. Andrews *,
Jie Na1,3*
1 Biomedical Science Department, Centre for Stem Cell Biology, University of Sheffield, Sheffield, United Kingdom, 2 Department of Biochemical Engineering, University
College London, London, United Kingdom, 3 School of Medicine, Tsinghua University, Beijing, China
Abstract
Background: Several methods have been used to induce somatic cells to re-enter the pluripotent state. Viral transduction of
reprogramming genes yields higher efficiency but involves random insertions of viral sequences into the human genome.
Although induced pluripotent stem (iPS) cells can be obtained with the removable PiggyBac transposon system or an
episomal system, both approaches still use DNA constructs so that resulting cell lines need to be thoroughly analyzed to
confirm they are free of harmful genetic modification. Thus a method to change cell fate without using DNA will be very
useful in regenerative medicine.
Methodology/Principal Findings: In this study, we synthesized mRNAs encoding OCT4, SOX2, cMYC, KLF4 and SV40 large T
(LT) and electroporated them into human fibroblast cells. Upon transfection, fibroblasts expressed these factors at levels
comparable to, or higher than those in human embryonic stem (ES) cells. Ectopically expressed OCT4 localized to the cell
nucleus within 4 hours after mRNA introduction. Transfecting fibroblasts with a mixture of mRNAs encoding all five factors
significantly increased the expression of endogenous OCT4, NANOG, DNMT3b, REX1 and SALL4. When such transfected
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