有哪些信誉好的足球投注网站a phosphate-regulated promoter for fine-tuned and reversible overexpression in ostreococcus application to circadian clock functional analysis调整phosphate-regulated启动子和可逆超表达ostreococcus应用生物钟功能分析.pdfVIP
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a phosphate-regulated promoter for fine-tuned and reversible overexpression in ostreococcus application to circadian clock functional analysis调整phosphate-regulated启动子和可逆超表达ostreococcus应用生物钟功能分析
A Phosphate-Regulated Promoter for Fine-Tuned and
Reversible Overexpression in Ostreococcus: Application
to Circadian Clock Functional Analysis
1,2. ´ ´ 1,2. 1,2 1,2
El Batoul Djouani-Tahri , Frederic Sanchez , Jean-Claude Lozano , Franc¸ois-Yves Bouget *
´ ´
1 Universite Pierre et Marie Curie (Paris 06), Observatoire Oceanologique, Banyuls/mer, France, 2 Centre national de la Recherche Scientifique, Unite Mixte de Recherche,
´
UMR7621, LOMIC, Laboratoire d’oceanographie microbienne, Banyuls/mer, France
Abstract
Background: The green picoalga Ostreococcus tauri (Prasinophyceae), which has been described as the smallest free-living
eukaryotic organism, has minimal cellular ultra-structure and a very small genome. In recent years, O. tauri has emerged as a
novel model organism for systems biology approaches that combine functional genomics and mathematical modeling, with
a strong emphasis on light regulated processes and circadian clock. These approaches were made possible through the
implementation of a minimal molecular toolbox for gene functional analysis including overexpression and knockdown
strategies. We have previously shown that the promoter of the High Affinity Phosphate Transporter (HAPT) gene drives the
expression of a luciferase reporter at high and constitutive levels under constant light.
Methodology/Principal Findings: Here we report, using a luciferase reporter construct, that the HAPT promot
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