有哪些信誉好的足球投注网站a pilot trial assessing urinary gene expression profiling with an mrna array for diabetic nephropathy一个试点试验评估尿基因表达分析糖尿病肾病的信使rna数组.pdfVIP
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a pilot trial assessing urinary gene expression profiling with an mrna array for diabetic nephropathy一个试点试验评估尿基因表达分析糖尿病肾病的信使rna数组
A Pilot Trial Assessing Urinary Gene Expression Profiling
with an mRNA Array for Diabetic Nephropathy
1. 1. 1 1 1 1 1 2
Min Zheng , Lin-Li Lv , Yu-Han Cao , Hong Liu , Jie Ni , Hou-Yong Dai , Dan Liu , Xiang-Dong Lei , Bi-
Cheng Liu1*
1 Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing, China, 2 CT Bioscience, Chang Zhou, China
Abstract
Background: The initiation and progression of diabetic nephropathy (DN) is complex. Quantification of mRNA expression in
urinary sediment has emerged as a novel strategy for studying renal diseases. Considering the numerous molecules
involved in DN development, a high-throughput platform with parallel detection of multiple mRNAs is needed. In this study,
we constructed a self-assembling mRNA array to analyze urinary mRNAs in DN patients with aims to reveal its potential in
searching novel biomarkers.
Methods: mRNA array containing 88 genes were fabricated and its performance was evaluated. A pilot study with 9 subjects
including 6 DN patients and 3 normal controls were studied with the array. DN patients were assigned into two groups
according to their estimate glomerular rate (eGFR): DNI group (eGFR.60 ml/min/1.73 m2, n = 3) and DNII group
(eGFR,60 ml/min/1.73 m2, n = 3). Urinary cell pellet was collected from each study participant. Relative abundance of these
target mRNAs from urinary pellet was quantified with the array.
Results: The array we fabricated displayed high sensitivity and specificity. Moreover, the Cts of Positive PCR Controls in our
experiments were 24 60.5 which indicated high repeatability of the array. A total of 29 mRNAs were significantly increased
in DN patients compared w
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