有哪些信誉好的足球投注网站a multi-platform flow device for microbial (co-) cultivation and microscopic analysis微生物多平台流装置(co)栽培和微观分析.pdfVIP
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a multi-platform flow device for microbial (co-) cultivation and microscopic analysis微生物多平台流装置(co)栽培和微观分析
A Multi-Platform Flow Device for Microbial (Co-)
Cultivation and Microscopic Analysis
Matthijn C. Hesselman1., Dorett I. Odoni 1., Brendan M. Ryback 1., Suzette de Groot 1, Ruben G. A. van
Heck1, Jaap Keijsers 1, Pim Kolkman 1, David Nieuwenhuijse 1, Youri M. van Nuland 1, Erik Sebus 1,
1 1 1 2 ¨ 3 ´
Rob Spee , Hugo de Vries , Marten T. Wapenaar , Colin J. Ingham , Karin Schroen , Vıtor A. P. Martins
dos Santos4, Sebastiaan K. Spaans 1,5, Floor Hugenholtz 1,5,6, Mark W. J. van Passel 1,4*
1 Wageningen UR iGEM 2011 Team, Wageningen University, Wageningen, The Netherlands, 2 MicroDish BV, Utrecht, The Netherlands, 3 Food Process Engineering,
Wageningen University, Wageningen, The Netherlands, 4 Systems and Synthetic Biology, Wageningen University, Wageningen, The Netherlands, 5 Laboratory of
Microbiology, Wageningen University, Wageningen, The Netherlands, 6 Netherlands Consortium for Systems Biology, University of Amsterdam, Amsterdam, The
Netherlands
Abstract
Novel microbial cultivation platforms are of increasing interest to researchers in academia and industry. The development of
materials with specialized chemical and geometric properties has opened up new possibilities in the study of previously
unculturable microorganisms and has facilitated the design of elegant, high-throughput experimental set-ups. Within the
context of the international Genetically Engineered Machine (iGEM) competition, we set out to design, manufacture, and
implement a flow device that can accommodate multiple growth platforms, that is, a silicon nitride based microsieve and a
porous aluminium oxide based microdish. It provides control over (co-)culturing conditions similar to
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