a major role for the plasmodium falciparum apiap2 protein pfsip2 in chromosome end biology恶性疟原虫的主要角色apiap2蛋白质pfsip2染色体生物学.pdfVIP

A Major Role for the Plasmodium falciparum ApiAP2 Protein PfSIP2 in Chromosome End Biology 1 2 1 1 2 Christian Flueck , Richard Bartfai , Igor Niederwieser , Kathrin Witmer , Blaise T. F. Alako , Suzette 3 4 3 2 1 Moes , Zbynek Bozdech , Paul Jenoe , Hendrik G. Stunnenberg , Till S. Voss * 1 Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, University of Basel, Basel, Switzerland, 2 Department of Molecular Biology, Nijmegen Center of Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands, 3 Biozentrum, University of Basel, Basel, Switzerland, 4 School of Biological Sciences, Nanyang Technological University, Singapore Abstract The heterochromatic environment and physical clustering of chromosome ends at the nuclear periphery provide a functional and structural framework for antigenic variation and evolution of subtelomeric virulence gene families in the malaria parasite Plasmodium falciparum. While recent studies assigned important roles for reversible histone modifications, silent information regulator 2 and heterochromatin protein 1 (PfHP1) in epigenetic control of variegated expression, factors involved in the recruitment and organization of subtelomeric heterochromatin remain unknown. Here, we describe the purification and characterization of PfSIP2, a member of the ApiAP2 family of putative transcription factors, as the unknown nuclear factor interacting specifically with cis-acting SPE2 motif arrays in subtelomeric domains. Interestingly, SPE2 is not bound by the full-length protein but rather by a 60kDa N-terminal domain, P