a biomechanical analysis of ventral furrow formation in the drosophila melanogaster embryo腹侧沟形成的生物力学分析果蝇胚胎.pdfVIP

A Biomechanical Analysis of Ventral Furrow Formation in the Drosophila Melanogaster Embryo 1 2 3 ˜ 4 5 5,6 Vito Conte , Florian Ulrich , Buzz Baum , Jose Munoz , Jim Veldhuis , Wayne Brodland , Mark Miodownik7* 1 Institute for Bioengineering of Catalonia, Barcelona, Spain, 2 Skirball Institute of Biomolecular Medicine, New York Hospital, New York, New York, United States of America, ` ` 3 Medical Research Council Laboratory for Molecular Cell Biology, University College London, London, United Kingdom, 4 Laboratori de Calcul Numeric, Department of ` Applied Mathematics III, Universitat Politecnica de Catalunya, Barcelona, Spain, 5 Department of Civil and Environmental Engineering, University of Waterloo, Waterloo, Canada, 6 Department of Biology, University of Waterloo, Waterloo, Canada, 7 Mechanical Engineering Department, University College London, London, United Kingdom Abstract The article provides a biomechanical analysis of ventral furrow formation in the Drosophila melanogaster embryo. Ventral furrow formation is the first large-scale morphogenetic movement in the fly embryo. It involves deformation of a uniform cellular monolayer formed following cellularisation, and has therefore long been used as a simple system in which to explore the role of mechanics in force generation. Here we use a quantitative framework to carry out a systematic perturbation analysis to determine the role of each of the active forces observed. The analysis confirms that ventral furrow invagination arises from