a built-in strategy for containment of transgenic plants creation of selectively terminable transgenic rice一个内置的遏制战略创造转基因植物的选择性有期限的转基因大米.pdfVIP

A Built-In Strategy for Containment of Transgenic Plants: Creation of Selectively Terminable Transgenic Rice 1. 1. 1. 1 1 2 1 Chaoyang Lin , Jun Fang , Xiaoli Xu , Te Zhao , Jiaan Cheng , Juming Tu , Gongyin Ye , Zhicheng Shen1* 1 Institute of Insect Sciences and State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, China, 2 Institute of Crop Sciences, Zhejiang University, Hangzhou, China Abstract Plant transgenic technology has been widely utilized for engineering crops for trait improvements and for production of high value proteins such as pharmaceuticals. However, the unintended spreading of commercial transgenic crops by pollination and seed dispersal is a major concern for environmental and food safety. Simple and reliable containment strategies for transgenes are highly desirable. Here we report a novel method for creating selectively terminable transgenic rice. In this method, the gene(s) of interest is tagged with a RNA interference cassette, which specifically suppresses the expression of the bentazon detoxification enzyme CYP81A6 and thus renders transgenic rice to be sensitive to bentazon, a herbicide used for rice weed control. We generated transgenic rice plants by this method using a new glyphosate resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene from Pesudomonas putida as the gene of interest, and demonstrated that these transgenic rice plants were highly sensitive to bentazon but tolerant to glyphosate, which is exactly the opposite of conventional rice. Field trial of these transgenic rice plants further confirmed that they can be selectively killed at 100% by one spray of ben