a high-temporal resolution technology for dynamic proteomic analysis based on 35s labelinghigh-temporal解决动态蛋白质组学分析技术基于35 s标签.pdfVIP

A High-Temporal Resolution Technology for Dynamic Proteomic Analysis Based on 35S Labeling Zhao Zhang., Jian Chen., Fuzheng Guo, Liren He, Yizhou Wu, Changqing Zeng, Xueyuan Xiao, Dacheng He* Universities’ Confederated Institute of Proteomics, Key laboratory for Cell Proliferation and Regulation Biology Ministry of Education, Beijing Normal University, Beijing, People’s Republic of China Abstract As more and more research efforts have been attracted to dynamic or differential proteomics, a method with high temporal resolution and high throughput is required. In present study, a 35S in vivo Labeling Analysis for Dynamic Proteomics (SiLAD) was designed and tested by analyzing the dynamic proteome changes in the highly synchronized A549 cells, as well as in the rat liver 2/3 partial hepatectomy surgery. The results validated that SiLAD technique, in combination with 2-Dimensional Electrophoresis, provided a highly sensitivity method to illustrate the non-disturbed endogenous proteins dynamic changes with a good temporal resolution and high signal/noise ratio. A significant number of differential proteins can be discovered or re-categorized by this technique. Another unique feature of SiLAD is its capability of quantifying the rate of protein expression, which reflects the cellular physiological turn points more effectively. Finally, the prescribed SiLAD proteome snapshot pattern could be potentially used as an exclusive symbol for characterizing each stage in well regulated biological processes. Citation: Zhang Z, Chen J, Guo F, He L, Wu Y, et al. (2008) A High-Temporal Resolution Technology for Dynamic Proteomic Analysis Based on 35S Labeling. PLoS ONE 3(8): e2991. doi:10.1371/journal.pone.0002991 ¨ Editor: Jorg Hoheisel, Deutsches Krebsforschungszentrum, Germany Received January 7, 2008; Accepted July 28, 2008; Published August 20, 200