a comparative transcriptomic analysis of uveal melanoma and normal uveal melanocyte比较转录组分析葡萄膜黑色素瘤和正常眼色素层的黑素细胞.pdfVIP

A Comparative Transcriptomic Analysis of Uveal Melanoma and Normal Uveal Melanocyte Jianhong An1,2., Haolei Wan3., Xiangtian Zhou1,2, Dan-Ning Hu1,4, Ledan Wang1,2, Lili Hao3,5, 1,2 1,2 1,2 1,2 3 3 1,2 Dongsheng Yan , Fanjun Shi , Zhonglou Zhou , Jiao Wang , Songnian Hu , Jun Yu *, Jia Qu * 1 School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical College, Wenzhou, China, 2 State Key Laboratory Cultivation Base and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, China, 3 CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China, 4 Tissue Culture Center, The New York Eye and Ear Infirmary, New York Medical College, New York, New York, United States of America, 5 Graduate University of Chinese Academy of Sciences, Beijing, China Abstract Background: Uveal melanoma is the most common primary intraocular tumor in adults in western countries. It is associated with very severe visual morbidity and may lead to distant metastases even after successful treatment of the primary tumor. In order to gain better insight into molecular mechanisms related to tumorigenesis and metastasis of uveal melanoma, we used next-generation sequencing technology (SOLiD, Life Technologies) to acquire global transcriptome alteration between posterior uveal melanoma cells and normal uveal melanocyte. Results: From mRNAs of the cultured uveal melanoma cells and normal uveal melanocytes, we annotated more than 7 7 3.7 610 and 2.7 610 sequencing tags based on human Ensembl databases, respectively. For detailed